Mohammadi Maryam, Najafi Houshang, Mohamadi Yarijani Zeynab, Vaezi Gholamhasan, Hojati Vida
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Tradit Complement Med. 2019 Jul 26;10(6):570-576. doi: 10.1016/j.jtcme.2019.07.002. eCollection 2020 Nov.
Renal ischemia-reperfusion is associated with inflammation and oxidative stress. As a major compound in black pepper, piperine has anti-inflammatory and anti-oxidative properties. In present study, the protective effects of oral administration of piperine in renal ischemia-reperfusion (IR) induced acute kidney injuries (AKI) were investigated.
Male Wistar rats received piperine (10 or 20 mg/kg.bw) or vehicle for 10 days. The artery and vein of both kidneys were then clamped for 30 min, followed by a 24-h reperfusion period. Concentrations of creatinine and urea-nitrogen in descending aorta blood were measured, and malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels were measured in kidney tissue to evaluate the oxidative stress. Inflammation was evaluated by measuring the TNF-α and ICAM-1 mRNA expression levels in renal cortical tissue using Real Time PCR method and counting leukocytes infiltration to interstitium. Further measured were tissue damages in H & E stained sections.
Renal IR reduced FRAP, while increasing the plasma concentrations of creatinine and urea-nitrogen, tissue MDA level, TNF-α and ICAM-1 mRNA expressions, leukocyte infiltration and histopathologic injuries. Piperine administration significantly reduced the plasma concentrations of creatinine and urea-nitrogen, expression of pro-inflammatory factors, oxidative stress and renal histopathologic injuries. It is to be noted that 20 mg/kg dose was more effective.
Our results suggest piperine protects the kidney against ischemia-reperfusion induced acute kidney injuries by its anti-inflammatory and anti-oxidative properties.
肾缺血再灌注与炎症和氧化应激相关。胡椒碱作为黑胡椒中的主要成分，具有抗炎和抗氧化特性。在本研究中，探讨了口服胡椒碱对肾缺血再灌注（IR）诱导的急性肾损伤（AKI）的保护作用。
雄性Wistar大鼠接受胡椒碱（10或20mg/kg体重）或赋形剂处理10天。然后夹闭双侧肾脏的动静脉30分钟，随后进行24小时再灌注。测量降主动脉血中肌酐和尿素氮的浓度，并测定肾组织中丙二醛（MDA）和铁还原/抗氧化能力（FRAP）水平以评估氧化应激。通过实时PCR法测量肾皮质组织中TNF-α和ICAM-1 mRNA表达水平并计数白细胞向间质的浸润来评估炎症。进一步测量苏木精和伊红染色切片中的组织损伤。
肾IR降低了FRAP，同时增加了血浆肌酐和尿素氮浓度、组织MDA水平、TNF-α和ICAM-1 mRNA表达、白细胞浸润和组织病理学损伤。给予胡椒碱显著降低了血浆肌酐和尿素氮浓度、促炎因子表达、氧化应激和肾组织病理学损伤。需要注意的是，20mg/kg剂量更有效。
我们的结果表明，胡椒碱通过其抗炎和抗氧化特性保护肾脏免受缺血再灌注诱导的急性肾损伤。
