Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Food Chem. 2024 Dec 1;460(Pt 1):140308. doi: 10.1016/j.foodchem.2024.140308. Epub 2024 Jul 1.
Effect of part differences on metabolite molecule alterations in refrigerated pork was investigated. A metabolomics methodology combined with chemometric analysis was successfully established to identify key compounds and their conversion pathways, including precursors and volatile metabolites, in the Longissimus lumborum as well as the breast and flank stored for 11 days. In total, 12 discriminative precursors were identified using the Short Time-series Expression Miner. In tandem with Random Forest and ANOVA analyses, nine volatile metabolites were identified as key compounds that could be attributed to differences in pork sections. Bidirectional orthogonal partial least squares analysis revealed a potential correlation between these key metabolites and discriminative precursors. Metabolic pathway enrichment analysis demonstrated that the primary metabolic process affected by variations in pork sections is linoleic acid metabolism, which participates in the metabolism of cysteine and glutamic acid to produce methoxy-phenyl-oxime. This study provides valuable insights into the identification of differential metabolites.
研究了部位差异对冷藏猪肉代谢物分子变化的影响。成功建立了一种代谢组学方法与化学计量学分析相结合的方法，以鉴定 11 天贮藏的腰肉、胸肉和肋肉中关键化合物及其转化途径，包括前体物和挥发性代谢物。使用短时序列表达挖掘技术，共鉴定出 12 个有区别的前体物。通过随机森林和 ANOVA 分析，确定了 9 种挥发性代谢物为关键化合物，这些化合物可归因于猪肉部位的差异。双向正交偏最小二乘分析显示，这些关键代谢物与有区别的前体物之间存在潜在的相关性。代谢途径富集分析表明，猪肉部位变化影响的主要代谢过程是亚油酸代谢，该代谢参与半胱氨酸和谷氨酸的代谢，产生甲氧基-苯-肟。本研究为差异代谢物的鉴定提供了有价值的见解。
