Hua C, Buferne M, Schmitt-Verhulst A M
Eur J Immunol. 1985 Oct;15(10):1029-33. doi: 10.1002/eji.1830151013.
A B cell hybridoma (Désiré-1) was derived which secreted and expressed at its cell surface immunoglobulin (Ig) specific for the antigen-specific T cell receptor (Ti) of an H-2Kb-specific alloreactive cytotoxic T lymphocyte (CTL) clone (KB5-C20). It was found that the CTL clone could lyse hybridoma Désiré-1, whereas it could not lyse hybridoma which expressed surface Ig (sIg) binding to other cell surface structures of clone KB5-C20 such as the H-2Kk molecule. Blocking of CTL-target cell interactions using monoclonal antibodies (mAb) indicated that the CTL-target cell interaction was inhibited with appropriate anti-H-2 mAb and by anti-Lyt-2 mAb when CTL-H-2Kb interaction was involved but not when CTL-sIg interaction was involved. The two types of interactions were inhibited by anti-LFA-1 mAb. The involvement of the CTL-Ti structure was necessary to obtain a lytic interaction between CTL and target cells, but a major histocompatibility complex product on the target cells did not need to be involved. Comparison of CTL-target cell inhibition with cold target cells or with anti-clonotypic mAb indicated that the Ti-sIg cellular interaction was of much higher apparent affinity than the Ti-H-2Kb cellular interaction. These results further suggest potential regulatory effects of CTL-B cell cross-idiotypic interactions.
获得了一种B细胞杂交瘤（Désiré-1），它能分泌并在细胞表面表达针对H-2Kb特异性同种异体反应性细胞毒性T淋巴细胞（CTL）克隆（KB5-C20）的抗原特异性T细胞受体（Ti）的免疫球蛋白（Ig）。发现CTL克隆能够裂解杂交瘤Désiré-1，而不能裂解表达与克隆KB5-C20的其他细胞表面结构（如H-2Kk分子）结合的表面Ig（sIg）的杂交瘤。使用单克隆抗体（mAb）阻断CTL与靶细胞的相互作用表明，当涉及CTL与H-2Kb的相互作用时，适当的抗H-2 mAb和抗Lyt-2 mAb可抑制CTL与靶细胞的相互作用，而当涉及CTL与sIg的相互作用时则不然。这两种相互作用均被抗LFA-1 mAb抑制。CTL-Ti结构的参与对于获得CTL与靶细胞之间的裂解相互作用是必要的，但靶细胞上的主要组织相容性复合体产物无需参与。将CTL与靶细胞的抑制作用与冷靶细胞或抗独特型mAb进行比较表明，Ti-sIg细胞间相互作用的表观亲和力远高于Ti-H-2Kb细胞间相互作用。这些结果进一步提示了CTL与B细胞交叉独特型相互作用的潜在调节作用。
