School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Anal Chim Acta. 2024 Aug 15;1317:342919. doi: 10.1016/j.aca.2024.342919. Epub 2024 Jun 29.
The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in widespread disease transmission, challenging the stability of global healthcare systems. Surface-enhanced Raman scattering (SERS) as an easy operation, fast, and low-cost technology illustrates a good potential in detecting SARS-CoV-2. In the study, one-step fabrication of gold-silver alloy nanoparticles (AuAgNPs) with adjustable metal proportions and diameters is employed as SERS substrates. The angiotensin-converting enzyme 2 (ACE2) functionalized AuAgNPs are applied as sensor surfaces to detect SARS-CoV-2 S protein. By optimizing the SERS substrates, ACE2/AuAgNPs illustrate higher performance in detecting the SARS-CoV-2 S protein with a limit of detection (LOD) of 10 fg/mL in both phosphate-buffered saline (PBS) and pharyngeal swabs solution (PSS). It also provides excellent reproducibility with a relative standard deviation (RSD) of 7.7 % and 7.9 %, respectively. This easily preparable and highly reproducible SERS substrate has good potential in the practical application of detecting SARS-CoV-2.
由严重急性呼吸系统综合症冠状病毒 2 型（SARS-CoV-2）引起的 2019 年冠状病毒病（COVID-19）导致了广泛的疾病传播，挑战了全球医疗保健系统的稳定性。表面增强拉曼散射（SERS）作为一种易于操作、快速且低成本的技术，在检测 SARS-CoV-2 方面显示出良好的潜力。在这项研究中，采用一步法制备了具有可调金属比例和直径的金银合金纳米粒子（AuAgNPs），用作 SERS 基底。血管紧张素转化酶 2（ACE2）功能化的 AuAgNPs 被用作传感器表面，以检测 SARS-CoV-2 S 蛋白。通过优化 SERS 基底，ACE2/AuAgNPs 在磷酸盐缓冲盐水（PBS）和咽拭子溶液（PSS）中的检测 SARS-CoV-2 S 蛋白的检测限（LOD）分别达到 10 fg/mL，表现出更高的性能。它还具有出色的重现性，相对标准偏差（RSD）分别为 7.7%和 7.9%。这种易于制备且重现性良好的 SERS 基底在 SARS-CoV-2 的实际检测中具有良好的应用潜力。
