Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Anal Chim Acta. 2024 Aug 15;1317:342904. doi: 10.1016/j.aca.2024.342904. Epub 2024 Jun 23.
Resveratrol, a natural polyphenol compound used as an ingredient in dietary supplements, and pharmaceuticals, has gained significant attention due to its potential health benefits. However, the accurate and sensitive determination of resveratrol in complex matrices remains a challenge. In this study, we propose the utilization of bimetallic porous Mn/Co oxide nanosheets (MnCoO-NSs) as catalysts for the colorimetric determination of resveratrol.
The bimetallic porous MnCoO-NSs were prepared through a facile one-stone-two-birds strategy. These nanosheets exhibited superior oxidase-mimicking activity, as evidenced by the catalytic oxidation of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB), producing a blue-colored oxTMB species with a prominent absorbance peak at 655 nm. The catalytic activity was promoted through the production of superoxide anion (O), which enhanced the affinity of MnCoO-NSs to the TMB molecules. Upon the addition of resveratrol, the oxidation process was inhibited, resulting in rapid fading of the blue color. This colorimetric sensing platform exhibited a linear response to resveratrol concentrations over the range of 2.2-87.6 μM, with a limit of detection of 0.210 μM. The method was further applied for the determination of resveratrol in different matrices including biological fluids, pharmaceuticals, and environmental water.
The utilization of these MnCoO-NSs offers a simple and cost-effective alternative to conventional analytical techniques for the determination of resveratrol. Their high sensitivity, selectivity, and stability enable accurate measurements of resveratrol in various complex matrices. This research has implications in areas such as pharmaceutical analysis, biomedical research, and environmental analysis, where the reliable determination of resveratrol is crucial for assessing its therapeutic potential and ensuring product quality.
白藜芦醇是一种天然多酚化合物，被用作膳食补充剂和药物的成分，由于其潜在的健康益处而受到广泛关注。然而，在复杂基质中准确、灵敏地测定白藜芦醇仍然是一个挑战。在这项研究中，我们提出利用双金属多孔 Mn/Co 氧化物纳米片（MnCoO-NSs）作为催化剂，用于比色法测定白藜芦醇。
通过简便的一石二鸟策略制备了双金属多孔 MnCoO-NSs。这些纳米片表现出优异的氧化酶模拟活性，通过显色底物 3,3',5,5'-四甲基联苯胺（TMB）的催化氧化来证明，生成具有显著吸收峰在 655nm 的蓝色氧化 TMB 物质。这种催化活性是通过产生超氧阴离子（O）来促进的，这增强了 MnCoO-NSs 与 TMB 分子的亲和力。当加入白藜芦醇时，氧化过程被抑制，导致蓝色迅速褪色。这种比色传感平台对白藜芦醇浓度在 2.2-87.6μM 范围内表现出线性响应，检测限为 0.210μM。该方法进一步应用于不同基质中白藜芦醇的测定，包括生物流体、药物和环境水。
这些 MnCoO-NSs 的利用为传统分析技术提供了一种简单且经济有效的替代方法，用于测定白藜芦醇。它们具有高灵敏度、选择性和稳定性，能够准确测量各种复杂基质中的白藜芦醇。这项研究在药物分析、生物医学研究和环境分析等领域具有重要意义，在这些领域中，准确测定白藜芦醇对于评估其治疗潜力和确保产品质量至关重要。
