College of Science, Sichuan Agricultural University, Xinkang Road, Yucheng District, Ya'an, 625014, People's Republic of China.
Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, People's Republic of China.
Mikrochim Acta. 2019 May 10;186(6):340. doi: 10.1007/s00604-019-3434-5.
Nanosheets (NSs; type ZIF-67) of a metal organic framework (MOF) that was prepared from 2-methylimidazole, manganese(II) and cobalt(II) were obtained by an ultrasonic hydrothermal method. Their Mn(II) doping reached as much as 11.3%. The NSs inherit high porosity, a large specific surface, and a large number of active sites. They display superior oxidase-mimicking activity and can catalyze the oxidation of tetramethylbenzidine (TMB) by molecular oxygen to form blue oxTMB. Glutathione (GSH) can reduce oxTMB, so that less blue oxTMB will be present. A simple and rapid method was established for the colorimetric determination of GSH and of the activity of GSH reductase (GR), best at a wavelength of 652 nm. The response to GSH drops linearly in the 0.1-25 μM concentration range. The activity of GR can be quantified in the 0.1 - 3 mU⋅mL activity range. The respective detection limits are 0.07 μM and 0.18 mU⋅mL. Graphical abstract Schematic presentation of colorimetric detection of glutathione and glutathione reductase activity by the oxidase-mimicking activity of Mn-Co nanosheets in a metal organic framework.
采用超声水热法制备了一种由 2-甲基咪唑、锰(II)和钴(II)制备的金属有机骨架纳米片（NSs；ZIF-67 型）。其 Mn(II)掺杂量高达 11.3%。纳米片继承了高孔隙率、大比表面积和大量的活性位点。它们表现出优异的过氧化物酶模拟活性，并能催化分子氧氧化四甲基联苯胺（TMB）生成蓝色 oxTMB。谷胱甘肽（GSH）可以还原 oxTMB，从而使较少的蓝色 oxTMB 存在。建立了一种简单快速的比色法测定 GSH 和谷胱甘肽还原酶（GR）活性的方法，最佳波长为 652nm。GSH 的响应在 0.1-25μM 浓度范围内呈线性下降。GR 的活性在 0.1-3mU·mL 活性范围内可定量。各自的检测限分别为 0.07μM 和 0.18mU·mL。
