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构建青霉素结合蛋白5和6基因均缺失的大肠杆菌突变体。

Construction of a mutant of Escherichia coli that has deletions of both the penicillin-binding protein 5 and 6 genes.

作者信息

Broome-Smith J K

出版信息

J Gen Microbiol. 1985 Aug;131(8):2115-8. doi: 10.1099/00221287-131-8-2115.

DOI:10.1099/00221287-131-8-2115
PMID:3903044
Abstract

A mutant of Escherichia coli has been constructed with deletions of the genes encoding penicillin-binding protein 5 (dacA) and penicillin-binding protein 6 (dacC). The construction of this mutant establishes that the complete loss of the two most abundant species of penicillin-binding proteins can be tolerated by E. coli. Moreover, the double deletion mutant had the same growth rate and morphology as an isogenic dacA+ dacC+ strain.

摘要

已构建出一株大肠杆菌突变体,其编码青霉素结合蛋白5(dacA)和青霉素结合蛋白6(dacC)的基因被缺失。该突变体的构建表明,大肠杆菌能够耐受这两种最丰富的青霉素结合蛋白种类的完全缺失。此外,双缺失突变体与同基因的dacA + dacC +菌株具有相同的生长速率和形态。

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