一个新的大肠杆菌细胞分裂基因，ftsK。
A new Escherichia coli cell division gene, ftsK.
作者信息
Begg K J, Dewar S J, Donachie W D
机构信息
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
出版信息
J Bacteriol. 1995 Nov;177(21):6211-22. doi: 10.1128/jb.177.21.6211-6222.1995.
Abstract
A mutation in a newly discovered Escherichia coli cell division gene, ftsK, causes a temperature-sensitive late-stage block in division but does not affect chromosome replication or segregation. This defect is specifically suppressed by deletion of dacA, coding for the peptidoglycan DD-carboxypeptidase, PBP 5. FtsK is a large polypeptide (147 kDa) consisting of an N-terminal domain with several predicted membrane-spanning regions, a proline-glutamine-rich domain, and a C-terminal domain with a nucleotide-binding consensus sequence. FtsK has extensive sequence identity with a family of proteins from a wide variety of prokaryotes and plasmids. The plasmid proteins are required for intercellular DNA transfer, and one of the bacterial proteins (the SpoIIIE protein of Bacillus subtilis) has also been implicated in intracellular chromosomal DNA transfer.
摘要
新发现的大肠杆菌细胞分裂基因ftsK中的一个突变，会导致细胞分裂出现温度敏感型后期阻滞，但不影响染色体复制或分离。dacA基因的缺失可特异性抑制这种缺陷，dacA编码肽聚糖DD-羧肽酶PBP 5。FtsK是一种大型多肽（147 kDa），由一个具有多个预测跨膜区域的N端结构域、一个富含脯氨酸-谷氨酰胺的结构域和一个具有核苷酸结合共有序列的C端结构域组成。FtsK与来自多种原核生物和质粒的一类蛋白质具有广泛的序列同源性。质粒蛋白是细胞间DNA转移所必需的，并且一种细菌蛋白（枯草芽孢杆菌的SpoIIIE蛋白）也与细胞内染色体DNA转移有关。
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Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure.Lrp是大肠杆菌中的一种主要调节蛋白，它能使DNA弯曲，并能组织高阶核蛋白结构的组装。
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