Chang Xiaoli, Wang Lijun, Sun Hongwei, Wang Zhen, Yang Zongbao, Chen Shaozong
College of Acupuncture and moxibustion, Shandong University of Traditional Chinese Medicine, Jinan, China.
Research Institute of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine, Jinan, China.
Neurogastroenterol Motil. 2024 Oct;36(10):e14874. doi: 10.1111/nmo.14874. Epub 2024 Jul 19.
The aim of this study was to investigate the frequency dependence of electroacupuncture (EA) in alleviating chronic visceral pain in patients with irritable bowel syndrome (IBS) and the differences in the gut microbiota and metabolites as potential mechanisms to explain frequency dependence.
A visceral hyperalgesia model was established by colorectal instillation of 2,4,6-trinitrobenzene sulfonic acid in rats, and EA treatment at 2/10 Hz, 2/50 Hz and 2/100 Hz was applied at ST25. Visceral sensation was quantified by the abdominal withdrawal reflex score and the area under the curve of the rectus abdominis electromyogram in response to colorectal distension. Ultrastructural morphological damage of colonic tissue of the rats was examined by transmission electron microscopy. 16S rRNA gene sequencing and 1H-nuclear magnetic resonance spectroscopy were applied to study the differences in the gut microbiota and to perform metabonomic profiling of the colonic tissue.
EA at ST25 at different frequencies attenuated chronic visceral pain, ultrastructural morphological damage to colonic tissue and disruption of the gut microbiota in IBS rats. The frequency of 2/100 Hz has more regulatory pathways than 2/10 Hz and 2/50 Hz. In addition, IBS rats exhibited colonic metabolic disorders, and pantothenate was significantly upregulated after EA treatment at different frequencies. Very low-density lipoprotein and 2-hydroxybutyrate were significantly increased in the 2/10 Hz group, while low density lipoprotein, very low-density lipoprotein, 2-hydroxybutyrate, methylmalonate and alpha-hydroxyisobutyric acid were significantly increased in the 2/100 Hz group.
EA at ST25 at different frequencies attenuated chronic visceral pain through different gut microbiota and metabolic pathways.
本研究旨在探讨电针（EA）缓解肠易激综合征（IBS）患者慢性内脏痛的频率依赖性，以及肠道微生物群和代谢产物的差异，作为解释频率依赖性的潜在机制。
通过向大鼠结肠内灌注2,4,6-三硝基苯磺酸建立内脏高敏模型，并在ST25处施加2/10Hz、2/50Hz和2/100Hz的电针治疗。通过腹部回撤反射评分和腹直肌肌电图对结肠扩张反应的曲线下面积来量化内脏感觉。通过透射电子显微镜检查大鼠结肠组织的超微结构形态损伤。应用16S rRNA基因测序和1H-核磁共振波谱研究肠道微生物群的差异，并对结肠组织进行代谢组学分析。
不同频率的ST25电针可减轻IBS大鼠的慢性内脏痛、结肠组织超微结构形态损伤和肠道微生物群紊乱。2/100Hz的频率比2/10Hz和2/50Hz具有更多的调节途径。此外，IBS大鼠表现出结肠代谢紊乱，不同频率电针治疗后泛酸显著上调。2/10Hz组极低密度脂蛋白和2-羟基丁酸显著增加，而2/100Hz组低密度脂蛋白、极低密度脂蛋白、2-羟基丁酸、甲基丙二酸和α-羟基异丁酸显著增加。
不同频率的ST25电针通过不同的肠道微生物群和代谢途径减轻慢性内脏痛。
