Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, No. 17, Chunhui Road, Laishan District, Yantai, 264003, Shandong, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Biotechnol Lett. 2024 Dec;46(6):1069-1083. doi: 10.1007/s10529-024-03507-x. Epub 2024 Jul 20.
Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.
A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD of ~ 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 ± 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.
A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.
利用趋磁细菌 AMB-1 开发简化的摇瓶发酵策略，并优化铁补充剂以实现高产磁小体，从而解决与磁小体获取相关的挑战。
使用标准实验室耗材和设备建立了 AMB-1 纯培养的可靠处理方法。随后，优化了培养基和铁补充剂以提高 AMB-1 磁小体的产量。mSLM 支持摇瓶发酵中更高的生物量积累，达到 OD 值约 0.7。柠檬酸铁和 EDTA-Fe（比例为 0.5:0.5，浓度为 0.4 mmol/L）的预混溶液稳定了 Fe 并显著提高了 AMB-1 的还原酶活性。然后使用优化的发酵策略进行初始体积为 15 L 的摇瓶发酵。经过两轮铁和营养物补充后，磁小体产量达到 185.7±9.5 mg/批（约 12 mg/L），这是我们所知的 AMB-1 摇瓶发酵产量的最高水平。
开发了一种高产磁小体的摇瓶发酵策略，消除了对生物反应器的需求，大大简化了磁小体获取的过程。
