Institut de minéralogie et de physique des milieux condensés, UMR 7590 CNRS, Université Pierre et Marie Curie, 4 Place Jussieu, 75005, Paris, France.
Appl Microbiol Biotechnol. 2012 Nov;96(3):663-70. doi: 10.1007/s00253-012-4199-5. Epub 2012 Jun 16.
The introduction of various iron-chelating agents to the Magnetospirillum magneticum strain AMB-1 bacterial growth medium stimulated the growth of M. magneticum strain AMB-1 magnetotactic bacteria and enhanced the production of magnetosomes. After 7 days of growth, the number of bacteria and the production of magnetosomes were increased in the presence of iron-chelating agents by factors of up to ∼2 and ∼6, respectively. The presence of iron-chelating agents also produced an increase in magnetosome size and chain length and yielded improved magnetosome heating properties. The specific absorption rate of suspensions of magnetosome chains isolated from M. magneticum strain AMB-1 magnetotactic bacteria, measured under the application of an alternating magnetic field of average field strength ∼20 mT and frequency 198 kHz, increased from ∼222 W/g(Fe) in the absence of iron-chelating agent up to ∼444 W/g(Fe) in the presence of 4 μM rhodamine B and to ∼723 W/g(Fe) in the presence of 4 μM EDTA. These observations were made at an iron concentration of 20 μM and iron-chelating agent concentrations below 40 μM.
向趋磁螺菌 AMB-1 细菌生长培养基中引入各种铁螯合剂刺激了趋磁螺菌 AMB-1 趋磁细菌的生长，并提高了磁小体的产量。在存在铁螯合剂的情况下，经过 7 天的生长，细菌数量增加了约 2 倍，磁小体的产量增加了约 6 倍。铁螯合剂的存在还增加了磁小体的尺寸和链长，并提高了磁小体的加热性能。在施加平均场强约 20 mT、频率为 198 kHz 的交变磁场的情况下，从趋磁螺菌 AMB-1 趋磁细菌分离出的磁小体链悬浮液的比吸收率从无铁螯合剂时的约 222 W/g(Fe)增加到 4 μM 若丹明 B 存在时的约 444 W/g(Fe)，并在 4 μM EDTA 存在时增加到约 723 W/g(Fe)。这些观察结果是在 20 μM 的铁浓度和低于 40 μM 的铁螯合剂浓度下进行的。
