Saydi Ali, Behpoor Naser, Khamis Abadi Fatemeh, Jung Friedrich, Kordi Negin
Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Department of Sport Physiology, Faculty of Human Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Clin Hemorheol Microcirc. 2024;88(3):373-384. doi: 10.3233/CH-242306.
This study aimed to investigate the effects of six weeks of continuous training and Nutrition Bio-shield (NBS) Superfood Supplementation on the state of oxidative stress by the expression of Nrf2, NOX4, superoxide dismutase, and malondialdehyde genes in the lungs of rats after methamphetamine withdrawal.
Forty male Wistar rats were randomly divided into five groups (n = 8, per group), undergoing methamphetamine administration (six weeks, 5 mg/kg ip, and once per day) followed by a 21-day withdrawal period. The rats were supplemented NBS superfood at a dosage of 25 g/kg per day for six weeks. The training protocol was 30 minutes of daily continuous training (treadmill running), five days a week for six weeks. The regimen escalated from a pace of 3 m/min for the initial 5 minutes, to 5 m/min for the following 5 minutes, culminating at 8 m/min for the remainder of the session, all at a 0° incline. A one-way analysis of variance was performed to analyze the gene expression of Nrf2, NOX4, MDA, and SOD in the lungs tissue of rats.
The results indicated that, in the experimental groups which underwent continuous training and NBS Superfood supplementation, the expression of the Nrf2 gene exhibited a significant elevation compared to the control group (P < 0.05), while the NOX4, MDA, and SOD genes expression exhibited a significant decline in comparison to the control group (P < 0.05).
In general, both exercise interventions and NBS superfood supplementation, when employed separately or in combination after methamphetamine withdrawal, can enhance the state of oxidative stress in the lung.
本研究旨在通过检测甲基苯丙胺戒断后大鼠肺组织中Nrf2、NOX4、超氧化物歧化酶和丙二醛基因的表达，探讨六周持续训练和营养生物护盾（NBS）超级食物补充剂对氧化应激状态的影响。
40只雄性Wistar大鼠随机分为五组（每组n = 8），接受甲基苯丙胺给药（六周，5mg/kg腹腔注射，每天一次），随后有21天的戒断期。大鼠每天以25g/kg的剂量补充NBS超级食物，持续六周。训练方案为每天进行30分钟的持续训练（跑步机跑步），每周五天，共六周。训练强度从最初5分钟的3m/分钟逐渐增加，接下来5分钟为5m/分钟，最后阶段为8m/分钟，全程坡度为0°。采用单因素方差分析来分析大鼠肺组织中Nrf2、NOX4、丙二醛和超氧化物歧化酶的基因表达。
结果表明，在接受持续训练和NBS超级食物补充剂的实验组中，Nrf2基因的表达与对照组相比显著升高（P < 0.05），而NOX4、丙二醛和超氧化物歧化酶基因的表达与对照组相比显著下降（P < 0.05）。
总体而言，甲基苯丙胺戒断后单独或联合使用运动干预和NBS超级食物补充剂，均可改善肺部的氧化应激状态。
