Rouholamini Fatemeh Sadat, Aminaei Mohsen, Aminizadeh Soheil
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Physiology Research Center, Institute of Neuropharmacology, Department of Physiology and Pharmacology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Exp Gerontol. 2024 Oct 15;196:112572. doi: 10.1016/j.exger.2024.112572. Epub 2024 Sep 4.
The present study aimed to investigate the effects of endurance training (ET) in combination with MitoQ supplementation on antioxidant indices and the expression of sesterin-2 (SESN2) as an anti-aging factor and AMPK as an energy sensor in aged male Wistar rats.
Twenty-eight aged Wistar rats (410 ± 15 g, 22 ± 1.5 months old) were randomly divided into four groups (n = 7): Control, ET (eight weeks endurance training on the treadmill), MitoQ (250 μ/L in drinking water), and ET + MitoQ. We measured the protein and gene expression of SESN2 and AMPK in the heart tissue by western blotting and real-time PCR, respectively. In addition, antioxidant indices, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity, and oxidant malondialdehyde (MDA) concentration in the cardiac tissue and serum were measured.
SESN2 and AMPK protein expression significantly increased in the MitoQ group compared to the control group (P = 0.002, P = 0.0003). MDA content in tissue and serum remained unchanged in all groups (P > 0.05). MitoQ supplementation significantly increased SOD and GPx enzyme activity in serum and cardiac tissue (P = 0.001).
Overall, ET and MitoQ alone and in combination have anti-aging effects and improve the expression of AMPK and SESN2. Additionally, ET and MitoQ lead to improved antioxidant capacity in aged rats by ameliorating the activity of antioxidant enzymes.
本研究旨在探讨耐力训练（ET）联合MitoQ补充剂对老年雄性Wistar大鼠抗氧化指标以及作为抗衰老因子的 sestrin-2（SESN2）和作为能量传感器的AMPK表达的影响。
将28只老年Wistar大鼠（410±15克，22±1.5月龄）随机分为四组（n = 7）：对照组、ET组（在跑步机上进行为期八周的耐力训练）、MitoQ组（饮用水中添加250μ/L MitoQ）和ET + MitoQ组。我们分别通过蛋白质印迹法和实时PCR检测心脏组织中SESN2和AMPK的蛋白质和基因表达。此外，还测量了心脏组织和血清中的抗氧化指标、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性以及氧化产物丙二醛（MDA）浓度。
与对照组相比，MitoQ组中SESN2和AMPK的蛋白质表达显著增加（P = 0.002，P = 0.0003）。所有组的组织和血清中MDA含量均保持不变（P > 0.05）。补充MitoQ显著提高了血清和心脏组织中的SOD和GPx酶活性（P = 0.001）。
总体而言，单独及联合使用ET和MitoQ均具有抗衰老作用，并能改善AMPK和SESN2的表达。此外，ET和MitoQ通过改善抗氧化酶的活性，提高了老年大鼠的抗氧化能力。
