分析谷胱甘肽 S-转移酶 mu 类 5 基因甲基化为低级别胶质瘤的预后指标。
Analysis of glutathione Stransferase mu class 5 gene methylation as a prognostic indicator in low-grade gliomas.
机构信息
Department of Neurosurgery, The Seventh Medical Centre of PLA General Hospital, Beijing, China.
Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing, China.
出版信息
Technol Health Care. 2024;32(6):3925-3942. doi: 10.3233/THC-231316.
BACKGROUND
Low-grade gliomas (LGG) are a variety of brain tumors that show different clinical outcomes. The methylation of the GSTM5 gene has been noted in the development of LGG, however, its prognostic importance remains uncertain.
OBJECTIVE
The objective of this study was to examine the correlation between GSTM5 DNA methylation and clinical outcomes in individuals diagnosed with LGG.
METHODS
Analysis of GSTM5 methylation levels in LGG samples was conducted using data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets. The overall survival based on GSTM5 methylation status was evaluated using Kaplan-Meier curves. The DNA methylation heatmap for particular CpG sites in the GSTM5 gene was visualized using the "pheatmap" R package.
RESULTS
The study analyzed that LGG tumors had higher levels of GSTM5 methylation than normal tissues. There was an inverse relationship discovered between GSTM5 expression and methylation. LGG patients with hypermethylation of GSTM5 promoter experienced a positive outcome. Age, grade, and GSTM5 methylation were determined as independent prognostic factors in LGG through both univariate and multivariate Cox regression analyses.
CONCLUSION
Methylation of GSTM5 DNA, specifically at certain CpG sites, is linked to a positive outlook in patients with LGG. Utilizing the "pheatmap" R package to visualize GSTM5 methylation patterns offers important information for identifying prognostic markers and therapeutic targets in low-grade gliomas.
背景
低级别胶质瘤（LGG）是一种表现出不同临床结果的脑肿瘤。GSTM5 基因的甲基化已在 LGG 的发生发展中被发现，但它的预后意义尚不确定。
目的
本研究旨在探讨 GSTM5 DNA 甲基化与诊断为 LGG 的个体临床结局之间的相关性。
方法
使用来自癌症基因组图谱（TCGA）和基因表达综合（GEO）数据集的数据，分析 LGG 样本中 GSTM5 甲基化水平。使用 Kaplan-Meier 曲线评估基于 GSTM5 甲基化状态的总生存率。使用“pheatmap”R 包可视化 GSTM5 基因中特定 CpG 位点的 DNA 甲基化热图。
结果
该研究分析发现，LGG 肿瘤的 GSTM5 甲基化水平高于正常组织。发现 GSTM5 表达与甲基化之间存在负相关关系。GSTM5 启动子高甲基化的 LGG 患者预后良好。通过单变量和多变量 Cox 回归分析，年龄、分级和 GSTM5 甲基化被确定为 LGG 的独立预后因素。
结论
GSTM5 DNA 的甲基化，特别是特定 CpG 位点的甲基化，与 LGG 患者的良好预后相关。使用“pheatmap”R 包可视化 GSTM5 甲基化模式，为鉴定低级别胶质瘤的预后标志物和治疗靶点提供了重要信息。
Zotero 插件