对一种大型RNA聚合酶III转录因子与一个tRNA基因结合的电子显微镜检查。
Electron-microscopic examination of the binding of a large RNA polymerase III transcription factor to a tRNA gene.
作者信息
Stillman D J, Better M, Geiduschek E P
出版信息
J Mol Biol. 1985 Sep 20;185(2):451-5. doi: 10.1016/0022-2836(85)90417-6.
PMID:3903164
Abstract
A Saccharomyces cerevisiae RNA polymerase III transcription factor was previously shown to bind stably to tRNA genes. This transcription factor has been further purified on the basis of its large size and its binding to a S. cerevisiae tRNALeu3 gene has been examined by electron microscopy. Site-specific binding of the factor to the tRNALeu3 gene sharply bends the DNA.
摘要
先前已证明一种酿酒酵母RNA聚合酶III转录因子能稳定结合于tRNA基因。该转录因子基于其较大的尺寸得到了进一步纯化，并且通过电子显微镜检查了它与酿酒酵母tRNALeu3基因的结合情况。该因子与tRNALeu3基因的位点特异性结合使DNA发生急剧弯曲。
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