Industry 4.0 Convergence Bionics Engineering, Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine-Integrated Biomedical Technology, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
Center for Advanced Models for Translational Sciences and Therapeutics and Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
J Photochem Photobiol B. 2024 Sep;258:112990. doi: 10.1016/j.jphotobiol.2024.112990. Epub 2024 Jul 19.
Photobiomodulation (PBM) has widely been used to effectively treat complications associated with cancer treatment, including oral mucositis, radiation dermatitis, and surgical wounds. However, the safety of PBM against cancer still needs to be validated as the effects of PBM on cancer cells and their mechanisms are unclear. The current study investigated the wavelength-dependent PBM effects by examining four different laser wavelengths (405, 532, 635, and 808 nm) on B16F10 melanoma tumor cells. In vitro tests showed that PBM with 808 nm promoted both proliferation and migration of B16F10 cells. In vivo results demonstrated that PBM with 808 nm significantly increased the relative tumor volume and promoted angiogenesis with overexpression of VEGF and HIF-1α. In addition, PBM induced the phosphorylation of factors closely related to cancer cell proliferation and tumor growth and upregulated the related gene expression. The current result showed that compared to the other wavelengths, 808 nm yielded a significant tumor-stimulating effect the malignant melanoma cancer. Further studies will investigate the in-depth molecular mechanism of PBM on tumor stimulation in order to warrant the safety of PBM for clinical cancer treatment.
光生物调节（PBM）已广泛用于有效治疗与癌症治疗相关的并发症，包括口腔黏膜炎、放射性皮炎和手术伤口。然而，PBM 对癌症的安全性仍需验证，因为 PBM 对癌细胞及其机制的影响尚不清楚。本研究通过检查四种不同的激光波长（405、532、635 和 808nm）对 B16F10 黑色素瘤肿瘤细胞，研究了波长依赖性的 PBM 效应。体外试验表明，808nm 的 PBM 促进了 B16F10 细胞的增殖和迁移。体内结果表明，808nm 的 PBM 显著增加了相对肿瘤体积，并通过过度表达 VEGF 和 HIF-1α 促进了血管生成。此外，PBM 诱导了与癌细胞增殖和肿瘤生长密切相关的因子的磷酸化，并上调了相关基因的表达。目前的结果表明，与其他波长相比，808nm 对恶性黑色素瘤产生了显著的肿瘤刺激作用。进一步的研究将探讨 PBM 对肿瘤刺激的深入分子机制，以保证 PBM 在癌症临床治疗中的安全性。
