Institute of Future Lighting, Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai 200433, China.
Institute of Future Lighting, Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai 200433, China; Zhongshan Fudan Joint Innovation Center, 6th Xiangxing Road, Zhongshan City 528403, China.
J Photochem Photobiol B. 2021 Mar;216:112127. doi: 10.1016/j.jphotobiol.2021.112127. Epub 2021 Jan 19.
Cutaneous melanoma is one of the aggressive cancers. Recent studies have shown that Photobiomodulation (PBM) can inhibit the proliferation of melanoma cells. However, it is not clear that the effect of PBM light mode on the inhibition of melanoma cells. Herein, we investigated the difference of influence between continuous wave (CW) and Pulse PBM on B16F10 melanoma cells. Our results suggested that Pulse mode had a more significant inhibition on the viability of B16F10 melanoma cells than CW mode under the PBM light parameter of wavelength, dose, and average irradiance at 457 nm, 1.14 J/cm, and 0.19 mW/cm. Besides, we revealed the differentially expressed genes of B16F10 melanoma cells under the various treatments of PBM light mode (not PBM treatment, CW mode, and Pulse mode) by RNA sequencing. Together, our data suggested that Pulse-PBM can improve the effect of PBM on cells significantly and there may be different molecular mechanisms between Pulse and CW mode including anti-proliferative and cell necrosis. The study shed new light on investigating the molecular mechanisms of various PBM light modes on B16F10 melanoma cells.
皮肤黑色素瘤是一种侵袭性癌症。最近的研究表明，光生物调节（PBM）可以抑制黑色素瘤细胞的增殖。然而，目前尚不清楚 PBM 光模式对黑色素瘤细胞抑制的影响。在此，我们研究了连续波（CW）和脉冲 PBM 对 B16F10 黑色素瘤细胞的影响差异。我们的结果表明，在波长、剂量和平均辐照度为 457nm、1.14J/cm 和 0.19mW/cm 的 PBM 光参数下，与 CW 模式相比，脉冲模式对 B16F10 黑色素瘤细胞活力的抑制作用更为显著。此外，我们通过 RNA 测序揭示了 B16F10 黑色素瘤细胞在不同 PBM 光模式（非 PBM 处理、CW 模式和脉冲模式）处理下的差异表达基因。总之，我们的数据表明，脉冲 PBM 可以显著提高 PBM 对细胞的效果，脉冲模式和 CW 模式之间可能存在不同的分子机制，包括抗增殖和细胞坏死。该研究为研究不同 PBM 光模式对 B16F10 黑色素瘤细胞的分子机制提供了新的思路。
