State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Bioorg Chem. 2024 Oct;151:107655. doi: 10.1016/j.bioorg.2024.107655. Epub 2024 Jul 17.
Given the escalating incidence of bacterial diseases and the challenge posed by pathogenic bacterial resistance, it is imperative to identify appropriate methodologies for conducting proteomic investigations on bacteria, and thereby promoting the target-based drug/pesticide discovery. Interestingly, a novel technology termed "activity-based protein profiling" (ABPP) has been developed to identify the target proteins of active molecules. However, few studies have summarized advancements in ABPP for identifying the target proteins in antibacterial-active compounds. In order to accelerate the discovery and development of new drug/agrochemical discovery, we provide a concise overview of ABPP and its recent applications in antibacterial agent discovery. Diversiform cases were cited to demonstrate the potential of ABPP for target identification though highlighting the design strategies and summarizing the reported target protein of antibacterial compounds. Overall, this review is an excellent reference for probe design towards antibacterial compounds, and offers a new perspective of ABPP in bactericide development.
鉴于细菌疾病的发病率不断上升以及致病细菌耐药性带来的挑战，有必要确定合适的方法来对细菌进行蛋白质组学研究，从而促进基于靶标的药物/农药发现。有趣的是，已经开发出一种称为“基于活性的蛋白质谱分析”（ABPP）的新技术来鉴定活性分子的靶蛋白。然而，很少有研究总结 ABPP 在鉴定抗菌活性化合物的靶蛋白方面的进展。为了加速新药/农药发现的发现和开发，我们提供了 ABPP 的简明概述及其在抗菌剂发现中的最新应用。引用了多种情况来说明 ABPP 用于靶标鉴定的潜力，同时强调了设计策略并总结了报道的抗菌化合物的靶蛋白。总的来说，这篇综述是针对抗菌化合物的探针设计的极好参考，并为杀菌剂开发中的 ABPP 提供了新的视角。
