Department of Entomology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
Environ Toxicol Pharmacol. 2024 Sep;110:104516. doi: 10.1016/j.etap.2024.104516. Epub 2024 Jul 18.
Partamona helleri is an important pollinator in the Neotropics. However, this bee faces an increased risk of pesticide exposure, potentially affecting both individual bees and entire colonies. Thus, this study aimed to evaluate the effects of the herbicide tebuthiuron on behavior, antioxidant activity, midgut morphology, and signaling pathways related to cell death, cell proliferation and differentiation in P. helleri workers. tebuthiuron significantly reduced locomotor activity and induced morphological changes in the midgut. The activity of the detoxification enzymes superoxide dismutase and glutathione S-transferase increased after exposure, indicating a detoxification mechanism. Furthermore, the herbicide led to alterations in the number of positive cells for signaling-pathway proteins in the midgut of bees, suggesting induction of apoptotic cell death and disruption of midgut epithelial regeneration. Therefore, tebuthiuron may negatively impact the behavior, antioxidant activity, morphology, and physiology of P. helleri workers, potentially posing a threat to the survival of this non-target organism.
赫勒氏蜡鳞蜂是新热带地区的重要传粉者。然而，这种蜜蜂面临着更高的接触农药风险，这可能会对个体蜜蜂和整个蜂群都产生影响。因此，本研究旨在评估除草剂特丁津对赫勒氏蜡鳞蜂工蜂的行为、抗氧化活性、中肠形态以及与细胞死亡、细胞增殖和分化相关的信号通路的影响。特丁津显著降低了工蜂的活动能力，并诱导了中肠的形态变化。接触后，解毒酶超氧化物歧化酶和谷胱甘肽 S-转移酶的活性增加，表明存在解毒机制。此外，除草剂导致蜜蜂中肠与信号通路蛋白呈阳性的细胞数量发生改变，提示诱导了细胞凋亡和破坏了中肠上皮细胞的再生。因此，特丁津可能会对赫勒氏蜡鳞蜂工蜂的行为、抗氧化活性、形态和生理产生负面影响，可能对这种非靶标生物的生存构成威胁。
