Department of Biomechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan; Department of Biochemical Science & Technology, College of Life Science, National Taiwan University, Taipei, Taiwan.
Department of Biomechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Sci Total Environ. 2024 Oct 20;948:174841. doi: 10.1016/j.scitotenv.2024.174841. Epub 2024 Jul 18.
This study demonstrates cellulose acetate (CA)-coated screen-printed carbon electrodes (SPCEs) for soil microbial activity detection. A capacitive sensor design utilizes a coated CA layer for effective insulation in electrolytes, eliminating the need for additional signal protection. Optimization involved comparing spin and dip coating methods, with a one-layer 10-second dip coating identified as the best balance between quality and yield. These CA/SPCEs exhibited remarkable stability over a month, suggesting their potential for long-term use in monitoring agricultural soils. Analysis of CA/SPCE profile and thickness provided insights into surface characteristics and the impact of the CA coating on electrode roughness. ATR-FTIR analysis, along with capacitive sensing, demonstrated superior sensitivity and precision for monitoring CA film degradation compared to mechanical gauges. Chemical degradation studies suggest CA's potential immunity in near-neutral environments, while enzymatic degradation investigations revealed dominance by enzymes, particularly in the initial stages. The CA/SPCE sensor responds to both enzymatic and chemical degradation, potentially serving as an indicator of total soil microbial activity. Soil experiments explored CA/SPCE with Cap-S for microbial activity sensing. Significant differences in the long-term degradation rate were observed in mycorrhizal fungi-enriched soil compared to controls, highlighting microbial influences. This study underscores the adaptability and versatility of this technology, particularly for assessing C-cycle microbial activity in agricultural fields.
本研究展示了醋酸纤维素（CA）涂层丝网印刷碳电极（SPCE）在土壤微生物活性检测中的应用。电容式传感器设计利用涂覆的 CA 层在电解质中实现有效绝缘，无需额外的信号保护。优化过程涉及比较旋转和浸涂方法，发现一层 10 秒的浸涂是在质量和产量之间取得最佳平衡的方法。这些 CA/SPCE 在一个月的时间内表现出显著的稳定性，表明它们在监测农业土壤方面具有长期使用的潜力。对 CA/SPCE 轮廓和厚度的分析提供了对表面特性和 CA 涂层对电极粗糙度影响的深入了解。ATR-FTIR 分析以及电容感应表明，与机械量具相比，CA 薄膜降解的监测具有更高的灵敏度和精度。化学降解研究表明 CA 在近中性环境中的潜在抗降解性，而酶降解研究表明酶的主导作用，尤其是在初始阶段。CA/SPCE 传感器对酶和化学降解都有响应，可能成为土壤微生物总活性的指示剂。土壤实验探索了 CA/SPCE 与 Cap-S 在微生物活性检测方面的应用。与对照相比，在富含菌根真菌的土壤中观察到长期降解率的显著差异，突出了微生物的影响。本研究强调了这项技术的适应性和多功能性，特别是在评估农业领域的 C 循环微生物活性方面。
