Department of Biomechatronics Engineering, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, Taiwan.
Department of Biomechatronics Engineering, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, Taiwan.
Biosens Bioelectron. 2024 Aug 15;258:116376. doi: 10.1016/j.bios.2024.116376. Epub 2024 May 9.
The capacitive immunosensor, known for its label-free simplicity, has great potential for point-of-care diagnostics. However, the interaction between insulation and recognition layers on the sensing electrode greatly affects its performance. This study introduces a pioneering dual-layer strategy, implementing a novel combination of acrylic resin (AR) and nitrocellulose (NC) coatings on screen-printed carbon electrodes (SPCEs). This innovative approach not only enhances the dielectric properties of the capacitive sensor but also streamlines the immobilization of recognizing elements. Particularly noteworthy is the superior reliability and insulation offered by the AR coating, surpassing the limitations of traditional self-assembled monolayer (SAM) modifications. This dual-layer methodology establishes a robust foundation for constructing capacitive sensors optimized specifically for liquid medium-based biosensing applications. The NC coating in this study represents a breakthrough in effectively immobilizing BSA, unraveling the capacitive response intricately linked to the quantity of adsorbed recognizing elements. The results underscore the prowess of the proposed immunosensor, showcasing a meticulously defined linear calibration curve for anti-BSA (ranging from 0 to 25 μg/ml). Additionally, specific interactions with anti-HAS and anti-TNF-α further validate the versatility and efficacy of the developed immunosensor. This work presents a streamlined and highly efficient protocol for developing label-free immunosensors for antibody determination and introduces a paradigm shift by utilizing readily available electrodes and sensing systems. The findings are poised to catalyze a significant acceleration in the advancement of biosensor technology, opening new avenues for innovative applications in point-of-care diagnostics.
基于其无标记简单性而闻名的电容式免疫传感器在即时诊断方面具有巨大潜力。然而，传感电极上的绝缘层和识别层之间的相互作用极大地影响了其性能。本研究引入了一种开创性的双层策略，在丝网印刷碳电极 (SPCE) 上实施了新型丙烯酸树脂 (AR) 和硝化纤维素 (NC) 涂层的组合。这种创新方法不仅增强了电容传感器的介电性能，而且简化了识别元件的固定化过程。特别值得注意的是 AR 涂层提供的卓越可靠性和绝缘性，克服了传统自组装单分子层 (SAM) 修饰的局限性。这种双层方法为构建专门针对基于液体介质的生物传感应用优化的电容传感器奠定了坚实的基础。本研究中的 NC 涂层在有效固定 BSA 方面取得了突破，揭示了与吸附识别元件数量密切相关的电容响应复杂性。研究结果突显了所提出的免疫传感器的强大功能，展示了针对抗 BSA（范围为 0 至 25μg/ml）的精心定义线性校准曲线。此外，与抗 HAS 和抗 TNF-α 的特异性相互作用进一步验证了所开发的免疫传感器的多功能性和有效性。本工作提出了一种用于开发用于抗体测定的无标记免疫传感器的简化且高效的方案，并通过利用现成的电极和传感系统引入了一种范式转变。研究结果有望显著加速生物传感器技术的发展，为即时诊断领域的创新应用开辟新途径。
