Cao Shulei, Wan Qiqi, Cao Ruihua, Wang Jingyi, Huang Tinglin, Wen Gang
Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, PR China.
Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, PR China.
Sci Total Environ. 2024 Oct 20;948:174886. doi: 10.1016/j.scitotenv.2024.174886. Epub 2024 Jul 18.
The risk of fungal pollution in drinking water has been paid attention. Solar/chlorine dioxide (ClO) combined system is an environment-friendly, economical and efficient disinfection method, especially for countries and regions that are economically backward and still exposed to unsafe drinking water. In this paper, the kinetics, influencing factors, mechanism and regrowth potential of inactivated Aspergillus niger (A. niger) spores by solar/ClO were reported for the first time. The inactivation curve can be divided into three stages: instant inactivation within 1-2 min, slow linear inactivation and finally a tail. The synergistic factors produced by solar/ClO in terms of log reduction and maximum inactivation rate were 1.194 and 1.112, respectively. The inhibitory effect on the regrowth of A. niger spores inactivated by solar/ClO was also stronger than that by ClO alone. Strongly oxidizing reactive species produced by solar/ClO accelerated the accumulation of endogenic reactive oxygen species (ROS) caused by oxidation stress of A. niger spores, improving the inactivation ability of the system. The inactivation order of A. niger spores was: loss of culturability, accumulation of intracellular ROS, loss of membrane integrity, leakage of intracellular species and change of morphology. The inactivation performance of solar/ClO was better than solar/chlor(am)ine according to the comparison of inactivation efficiency and regrowth potential. Results also suggested that solar/ClO process was more suitable for the treatment of ground water sources.
饮用水中真菌污染的风险已受到关注。太阳能/二氧化氯（ClO）联合系统是一种环境友好、经济高效的消毒方法，尤其适用于经济落后且仍面临不安全饮用水问题的国家和地区。本文首次报道了太阳能/ClO对黑曲霉（A. niger）孢子的灭活动力学、影响因素、机制及再生长潜力。灭活曲线可分为三个阶段：1 - 2分钟内的瞬间灭活阶段、缓慢的线性灭活阶段以及最后的拖尾阶段。太阳能/ClO在对数减少量和最大灭活率方面产生的协同因子分别为1.194和1.112。太阳能/ClO对黑曲霉孢子再生长的抑制作用也强于单独使用ClO。太阳能/ClO产生的强氧化性活性物种加速了黑曲霉孢子氧化应激引起的内源性活性氧（ROS）积累，提高了系统的灭活能力。黑曲霉孢子的灭活顺序为：可培养性丧失、细胞内ROS积累、膜完整性丧失、细胞内物质泄漏以及形态变化。根据灭活效率和再生长潜力的比较，太阳能/ClO的灭活性能优于太阳能/氯（胺）。结果还表明，太阳能/ClO工艺更适合处理地下水源。
