Heat moisture treatment and ultrasound-induced hydrothermal wheat starch modification: Techno-functional, microstructural and quality 3D printed characteristics.

In this study, the effect of hydrothermal process, ultrasound and combined freezing-ultrasound process on the physical and structural characteristics of wheat starch (Triticum aestivum) was investigated. Two heat-moisture treatments for 2 h (HMT2) and 4 h (HMT4), high-intensity, high-frequency ultrasound under two treatment times (10 to 20 min) (UT10 and UT20) as pre-treatment and sonication after freezing as post-treatment (FUT) on wheat starch suspension was applied. The modifications of starch crystallinity, chemical bonds of starch treated, morphology, thermal, swelling, pasting, and physicochemical characteristics were evaluated. Finally, the starches treated under these conditions were used as ink for a 3D printer, and the characteristics of the printed product were evaluated. The results demonstrate that heat-moisture modified starch increased swelling and size of granules and lowered syneresis values. Sonication promoted molecular depolymerization and reduction of starch swelling and crystallinity. Combined treatment (Sonication and freezing) showed higher peak apparent viscosity during gelatinization and pasting, and the FUT starch-based hydrogels showed the best printability (better ability to stack layers on top of each other and build the desired 3D shape), indicating better reproducibility of this ink. These results showed that FUT is a suitable process for improving the synergy and properties of wheat starch-based hydrogels, which are suitable as inks for use in 3D printers.