Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410013, China.
Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China.
Arch Microbiol. 2024 Jul 20;206(8):359. doi: 10.1007/s00203-024-04082-5.
In this experiment, the eutrophication system was established by adding sucrose and yeast powder, and the pH and dissolved oxygen were measured in a bioreactor in real time to study the effect of aerobic environment on the fermentation process of Polygonati Rhizoma extract by Lactiplantibacillus plantarum. To further analyze metabolic changes, UPLC-Q-Exactive MS was used for metabolomic analysis and metabolic profiling. Multivariate analysis was performed using principal component analysis and Orthogonal projections to latent structures discriminant analysis. Finally, 313 differential metabolites were selected, 196 of which were annotated through database matching. After fermentation, the content of short-chain fatty acids, lactic acid, and their derivatives increased significantly, and there were 13 kinds and 4 kinds, respectively. Both compounds and their derivatives are beneficial to the intestinal flora. Consequently, incorporating L. plantarum into the aerobic fermentation process of Polygonati Rhizoma extract within the eutrophic system is potentially advantageous in enhancing the impact of its fermentation solution on the gut microbiota and its effects on human health. Our findings for this kind of edible and medicinal material research and development offer useful insights.
在本实验中，通过添加蔗糖和酵母粉建立了富营养化体系，在生物反应器中实时测量 pH 值和溶解氧，研究好氧环境对植物乳杆菌发酵黄精提取物的发酵过程的影响。为了进一步分析代谢变化，采用 UPLC-Q-Exactive MS 进行代谢组学分析和代谢轮廓分析。采用主成分分析和正交偏最小二乘判别分析进行多变量分析。最终选择了 313 个差异代谢物，通过数据库匹配注释了其中的 196 个。发酵后，短链脂肪酸、乳酸及其衍生物的含量显著增加，分别有 13 种和 4 种化合物及其衍生物，均有利于肠道菌群。因此，在富营养化体系中，将植物乳杆菌纳入黄精提取物的好氧发酵过程中，可能有利于增强其发酵液对肠道微生物群的影响及其对人体健康的作用。本研究为这类药食同源的研究与开发提供了有益的见解。
