携带pKM101质粒的大肠杆菌uvrD、uvrE和recL菌株中的紫外线防护与诱变作用
UV protection and mutagenesis in uvrD, uvrE and recL strains of Escherichia coli carrying the pKM101 plasmid.
作者信息
Todd P A, Glickman B W
出版信息
Mutat Res. 1979 Oct;62(3):451-7. doi: 10.1016/0027-5107(79)90040-x.
PMID:390378
Abstract
The effect of the pKM101 plasmid on UV mutagenesis and survival was examined in DNA-repair-deficient strains of E. coli carrying the uvrD, uvrE and recL mutations. Although enhancement of UV mutagenesis by pKM101 was found in all 3 strains, UV protection was only observed in the uvrD strain. We conclude that the plasmid not only requires lexA+ recA+ functions of the cell, but also those of uvrE+ recL+ for its UV-protective effect.
摘要
在携带uvrD、uvrE和recL突变的大肠杆菌DNA修复缺陷菌株中，检测了pKM101质粒对紫外线诱变和存活的影响。尽管在所有3种菌株中都发现pKM101增强了紫外线诱变，但仅在uvrD菌株中观察到紫外线保护作用。我们得出结论，该质粒不仅需要细胞的lexA + recA +功能，还需要uvrE + recL +功能才能发挥其紫外线保护作用。
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