Laboratory of Molecular Immunology, Federal State Budgetary Scientific Institution Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia.
Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia.
PLoS One. 2024 Jul 22;19(7):e0305816. doi: 10.1371/journal.pone.0305816. eCollection 2024.
Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.
红细胞作为负责氧气运输的红细胞的祖细胞和前体细胞，表现出免疫抑制和免疫调节表型。我们研究小组的先前研究揭示了鼠骨髓红细胞中存在抗菌基因表达谱，这表明红细胞在先天免疫中发挥作用。在本研究中，我们通过综合分析（包括利用免疫反应 V2 面板进行的 NanoString 基因表达谱分析、趋化因子和 TGF-β家族蛋白分泌的 BioPlex 检查以及分析公开的单细胞 RNA-seq 数据），重点阐明人骨髓红细胞的特征。我们的研究结果表明，红细胞亚群表现出类似于髓样的基因表达特征，包括抗菌免疫和嗜中性粒细胞趋化基因，这表明人类红细胞参与先天免疫。此外，我们发现人类红细胞分泌 CCL22、CCL24、CXCL5、CXCL8 和 MIF 趋化因子。人类红细胞表达这些趋化因子的能力可能有助于在正常情况下限制骨髓中的免疫细胞，或者有助于红细胞在骨髓外造血时通过招募其附近的免疫细胞来诱导局部免疫抑制的能力。
