Perik-Zavodskii Roman, Perik-Zavodskaia Olga, Shevchenko Julia, Nazarov Kirill, Gizbrekht Anastasia, Alrhmoun Saleh, Denisova Vera, Sennikov Sergey
Laboratory of Molecular Immunology, Research Institute of Fundamental and Clinical Immunology, Novosibirsk 630099, Russia.
Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology, Novosibirsk 630099, Russia.
Biomedicines. 2025 May 17;13(5):1218. doi: 10.3390/biomedicines13051218.
: Recent studies have shown that Erythroid progenitor cells exhibit a distinct immunosuppressive and immunoregulatory phenotype associated with the response to bacteria. : The objective of this study was to comprehensively explore the traits of human bone marrow Erythroid cells through protein-protein interaction network analysis using cytokine secretion analysis, and single-cell immunoproteomic analysis using flow cytometry, as well as the re-analysis of publicly available human and mouse bone marrow Erythroid-cell transcriptomic data. : Our protein-protein interaction network analysis of human bone marrow Erythroid-cell protein-coding genes identified enrichment in the immune response to lipopolysaccharide, with Calprotectin and Cathepsin G being the main factors. We then mapped the Calprotectin to the CD45 Erythroid cells of both humans and mice via the analysis of the publicly available scRNA-seq data. Additionally, we observed that human bone marrow Erythroid cells secrete cytokines and chemokines, such as IL-1b, IL-8, and IL-18, which are also mainly involved in the immune response to lipopolysaccharide. We also found that human and mouse bone marrow Erythroid-cell conditional media inhibit bacterial growth in vitro. : These findings suggest that both human and mouse bone marrow CD45 Erythroid cells possess the potential to combat pathogenic microbes and thus play a role in innate antimicrobial immunity. : CD45 Erythroid cells are a potent immunoregulatory cell population in both humans and mice.
: 最近的研究表明，红系祖细胞表现出与对细菌的反应相关的独特免疫抑制和免疫调节表型。: 本研究的目的是通过使用细胞因子分泌分析的蛋白质-蛋白质相互作用网络分析、使用流式细胞术的单细胞免疫蛋白质组分析以及对公开可用的人类和小鼠骨髓红系细胞转录组数据的重新分析，全面探索人类骨髓红系细胞的特征。: 我们对人类骨髓红系细胞蛋白质编码基因的蛋白质-蛋白质相互作用网络分析确定了在对脂多糖的免疫反应中富集，其中钙卫蛋白和组织蛋白酶G是主要因素。然后，我们通过对公开可用的scRNA-seq数据的分析，将钙卫蛋白定位到人类和小鼠的CD45红系细胞上。此外，我们观察到人类骨髓红系细胞分泌细胞因子和趋化因子，如IL-1β、IL-8和IL-18，它们也主要参与对脂多糖的免疫反应。我们还发现人类和小鼠骨髓红系细胞条件培养基在体外抑制细菌生长。: 这些发现表明，人类和小鼠骨髓CD45红系细胞都具有对抗病原微生物的潜力，因此在先天性抗菌免疫中发挥作用。: CD45红系细胞在人类和小鼠中都是一种强大的免疫调节细胞群体。
