Gachogo Rachael, Happel Anna-Ursula, Alinde Berenice, Gray Clive M, Jaspan Heather, Dzanibe Sonwabile
Division of Immunology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
iScience. 2024 Jun 15;27(7):110282. doi: 10.1016/j.isci.2024.110282. eCollection 2024 Jul 19.
Infants who are HIV exposed but uninfected (iHEU) have higher risk of viral infections compared to infants who are HIV unexposed (iHUU). We explored the effect of intrauterine HIV exposure on the infant antibody repertoire by quantifying plasma immunoglobulin (Ig) G against 206 eukaryote-infecting viruses using phage immunoprecipitation sequencing (PhiPSeq) in iHEU and iHUU at birth and 36 weeks of life. Maternal HIV infection altered the infant IgG repertoire against eukaryote-infecting viruses at birth, resulting in significantly lower antibody breadth and diversity among iHEU compared to iHUU. Neonatal anti-viral IgG repertoire was dominated by antibodies against viruses belonging to the Herpesviridae family, although, by 36 weeks, this had shifted toward antibodies against enteroviruses, likely due to waning of maternal-derived antibodies and polio vaccine-induced antibody responses as expected. The observed reduced anti-viral IgG repertoire breadth and diversity acquired at birth in iHEU might contribute to the increased rates of viral infections among iHEU during early life.
与未接触过HIV的婴儿（iHUU）相比，接触过HIV但未感染的婴儿（iHEU）发生病毒感染的风险更高。我们通过使用噬菌体免疫沉淀测序（PhiPSeq）对出生时和出生36周时的iHEU和iHUU血浆中针对206种感染真核生物的病毒的免疫球蛋白（Ig）G进行定量，探讨了宫内HIV暴露对婴儿抗体库的影响。母亲的HIV感染改变了婴儿出生时针对感染真核生物病毒的IgG库，导致iHEU与iHUU相比抗体广度和多样性显著降低。新生儿抗病毒IgG库以针对疱疹病毒科病毒的抗体为主，不过到36周时，这已转向针对肠道病毒的抗体，这可能是由于母体来源抗体的减少以及如预期的脊髓灰质炎疫苗诱导的抗体反应。在iHEU中观察到的出生时获得的抗病毒IgG库广度和多样性降低，可能导致iHEU在生命早期病毒感染率增加。
