Liu Guoqing, Wang Min, Lv Xiangwen, Guan Yuting, Li Jingqi, Xie Jian
Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Guangxi Cardiovascular Institute, Nanning, Guangxi, China.
Department of Cardiology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
iScience. 2024 Jun 14;27(7):110275. doi: 10.1016/j.isci.2024.110275. eCollection 2024 Jul 19.
Mitochondrial dysfunction has been known to contribute to the worsening of acute myocardial infarction (AMI). We screened differentially expressed genes (DEGs) between AMI and healthy individuals based on the GSE66360 dataset. We took the intersection of the obtained DEGs with 1,136 mitochondria-related genes. Finally, we screened out mitochondria-related DEGs (MitoDEGs). Eight MitoDEGs were identified as hub genes based on the random forest algorithm. Two mitochondria-related robust molecular clusters were identified by consensus clustering. Immune infiltration analysis showed that immune cell infiltration was significantly increased in the high-expression group of MitoDEGs. We obtained the potential drugs targeted at , , and , such as disulfiram, obatoclax mesylate, and bortezomib. Quantitative reverse-transcription polymerase chain reaction further validated the expression of the MitoDEGs in the cell model of AMI. These findings reveal the potential role of MitoDEGs in AMI and provide new insights into risk stratification and individualized treatment of AMI patients.
已知线粒体功能障碍会导致急性心肌梗死（AMI）病情恶化。我们基于GSE66360数据集筛选了AMI患者与健康个体之间的差异表达基因（DEG）。我们将获得的DEG与1136个线粒体相关基因进行了交集分析。最后，我们筛选出线粒体相关DEG（MitoDEG）。基于随机森林算法，鉴定出8个MitoDEG作为枢纽基因。通过一致性聚类鉴定出两个线粒体相关的稳健分子簇。免疫浸润分析表明，MitoDEG高表达组的免疫细胞浸润显著增加。我们获得了针对 、 和 的潜在药物，如双硫仑、甲磺酸 obatoclax和硼替佐米。定量逆转录聚合酶链反应进一步验证了MitoDEG在AMI细胞模型中的表达。这些发现揭示了MitoDEG在AMI中的潜在作用，并为AMI患者的风险分层和个体化治疗提供了新的见解。
