Centro de Estudios en Ciencias de la Salud y la Enfermedad, Facultad de Odontología, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Mexico.
Centro de Investigación Multidisciplinaria Facultad de Medicina-UNAM-UABJO, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Mexico.
Acta Biochim Pol. 2024 Jul 8;71:13004. doi: 10.3389/abp.2024.13004. eCollection 2024.
CD36 is a type 2 cell surface scavenger receptor expressed in various tissues. In macrophages, CD36 recognizes oxidized low-density lipoprotein (ox-LDL), which promotes the formation of foam cells, the first step toward an atherosclerotic arterial lesion. CD36 possesses a variety of posttranslational modifications, among them N-glycosylation and O-GlcNAc modification. Some of the roles of these modifications on CD36 are known, such as N-linked glycosylation, which provides proper folding and trafficking to the plasma membrane in the human embryonic kidney. This study aimed to determine whether variations in the availability of UDP-GlcNAc could impact Rab-5-mediated endocytic trafficking and, therefore, the cellular localization of CD36. These preliminary results suggest that the availability of the substrate UDP-GlcNAc, modulated in response to treatment with Thiamet G (TMG), OSMI-1 (O-GlcNAcylation enzymes modulators) or Azaserine (HBP modulator), influences the localization of CD36 in J774 macrophages, and the endocytic trafficking as evidenced by the regulatory protein Rab-5, between the plasma membrane and the cytoplasm.
CD36 是一种表达在各种组织中的 2 型细胞表面清道夫受体。在巨噬细胞中，CD36 识别氧化型低密度脂蛋白（ox-LDL），促进泡沫细胞的形成，这是动脉粥样硬化病变的第一步。CD36 具有多种翻译后修饰，包括 N-糖基化和 O-GlcNAc 修饰。这些修饰中有些已知的作用，例如 N 连接的糖基化，它为人类胚胎肾中的质膜提供适当的折叠和运输。本研究旨在确定 UDP-GlcNAc 的可用性变化是否会影响 Rab-5 介导的内吞运输，从而影响 CD36 的细胞定位。这些初步结果表明，UDP-GlcNAc 的可用性可调节，以响应噻唑烷二酮 G（TMG）、OSMI-1（O-GlcNAc 修饰酶调节剂）或偶氮丝氨酸（HBP 调节剂）的处理，影响 J774 巨噬细胞中 CD36 的定位，以及内吞运输，如调节蛋白 Rab-5 所示，在质膜和细胞质之间。
