Fang Ke-Er, Zhang Hai-Rui, Zhu Bing, Ye Yu, Chen Lei, DU Wei-Feng, Ge Wei-Hong
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University Hangzhou 311402, China Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University Hangzhou 311401, China.
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University Hangzhou 311402, China Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University Hangzhou 311401, China Zhejiang Chinese Medical University Chinese Medicine Yinpian Co., Ltd. Hangzhou 311401, China.
Zhongguo Zhong Yao Za Zhi. 2024 Jun;49(12):3220-3228. doi: 10.19540/j.cnki.cjcmm.20240325.302.
This paper aims to study the spectrum-effect relationship between the fingerprints before and after salt processing of Dipsacus asper and the efficacy of warming and tonifying kidney Yang and find the main active components against kidney Yang deficiency before and after salt processing of D. asper, so as to provide the basis for clarifying the effect of salt processing on kidney Yang deficiency. The HPLC fingerprint before and after salt processing of D. asper was established by the HPLC-DAD. 15 common peaks were obtained, and 11 components were identified. The content changes of various components in rat serum were detected, and the difference in efficacy before and after salt processing was compared. The results of pharmacological experiments showed that salt processing of D. asper could enhance the kidney index. At the same dose, there was a significant difference between the raw D. asper and D. asper after salt processing groups. Compared with the model group, the contents of ACTH, cAMP, CORT, E_2, GH, Na+-K+-ATPase, T, and T4 in the serum of rats in the administration group increased to a certain extent, and the contents of cGMP and TNF-α decreased to a certain extent. Among them, there were significant differences in the above indexes in the serum of rats in the high-dose group of raw D. asper, middle-dose group of D. asper after salt processing, high-dose group of D. asper after salt processing, and the positive drug group. The overall results showed that D. asper after salt processing was more effective than raw D. asper in preventing kidney yang deficiency. The efficacy of D. asper was evaluated by grey correlation analysis, entropy method, and Pearson correlation analysis, and the components of D. asper after salt processing against kidney yang deficiency were screened out. According to the results of correlation degree ranking, the components with increased ranking before and after salt processing of D. asper were loganin, chlorogenic acid, dipsacoside A, asperosaponin Ⅵ, caffeic acid, and isochlorogenic acid B. It was preliminarily speculated that these compounds may be the potential pharmacodynamic components for the treatment of kidney yang deficiency before and after salt processing of D. asper. The changing components before and after the salt processing of D. asper were determined, which proved that D. asper after salt processing was superior to D. asper in the treatment of kidney yang deficiency. The spectrum-effect relationship between the efficacy of D. asper before and after salt processing and the treatment of kidney yang deficiency was established, which laid a foundation for the subsequent study on the pharmacodynamic components and molecular mechanism of salt processing of D. asper.
本文旨在研究盐制续断前后指纹图谱与温补肾阳功效的谱效关系，找出续断盐制前后抗肾阳虚的主要活性成分，为阐明盐制对肾阳虚的作用提供依据。采用高效液相色谱-二极管阵列检测器（HPLC-DAD）建立续断盐制前后的HPLC指纹图谱。共得到15个共有峰，鉴定出11个成分。检测大鼠血清中各成分的含量变化，比较盐制前后的药效差异。药理实验结果表明，盐制续断可提高肾脏指数。在相同剂量下，续断生品组与盐制续断组有显著差异。与模型组比较，给药组大鼠血清中促肾上腺皮质激素（ACTH）、环磷酸腺苷（cAMP）、皮质酮（CORT）、雌二醇（E₂）、生长激素（GH）、钠钾ATP酶（Na⁺-K⁺-ATPase）、睾酮（T）、甲状腺素（T4）含量有不同程度升高，环磷酸鸟苷（cGMP）、肿瘤坏死因子-α（TNF-α）含量有不同程度降低。其中，续断生品高剂量组、盐制续断中剂量组、盐制续断高剂量组大鼠血清上述指标与阳性药物组比较有显著差异。总体结果表明，盐制续断在防治肾阳虚方面优于续断生品。通过灰色关联分析、熵值法和Pearson相关分析对续断的药效进行评价，筛选出盐制续断抗肾阳虚的成分。根据关联度排序结果，续断盐制前后排序增加的成分有马钱苷、绿原酸、续断苷A、川续断皂苷Ⅵ、咖啡酸、异绿原酸B。初步推测这些化合物可能是续断盐制前后治疗肾阳虚的潜在药效成分。确定了续断盐制前后的变化成分，证明盐制续断在治疗肾阳虚方面优于续断生品。建立了续断盐制前后药效与治疗肾阳虚的谱效关系，为后续续断盐制药效成分及作用机制研究奠定了基础。
