腺病毒40和41基因组左末端区域导致的细胞转化

Cell transformation by the left terminal regions of the adenovirus 40 and 41 genomes.

作者信息

van Loon A E, Maas R, Vaessen R T, Reemst A M, Sussenbach J S, Rozijn T H

出版信息

Virology. 1985 Nov;147(1):227-30. doi: 10.1016/0042-6822(85)90245-4.

Abstract

To study the transforming capacity of the two fastidious adenoviruses 40 and 41 (Ad40 and Ad41), we have cloned the left terminal regions of their genomes. Transfection with plasmids containing these regions leads to transformation of primary baby rat kidney (BRK) cells. Cell lines derived from transformed cells show an intermediate transformed phenotype. The left terminal region was present in at least 50 copies in cells transformed by Ad41 and in 2-3 copies in Ad40-transformed cells. The integration patterns of the plasmids containing the left terminal region of Ad41 were similar in three different cell lines.

摘要

为研究两种苛养腺病毒40型和41型（Ad40和Ad41）的转化能力，我们克隆了它们基因组的左末端区域。用含有这些区域的质粒进行转染可导致原代幼鼠肾（BRK）细胞发生转化。源自转化细胞的细胞系表现出中间转化表型。在Ad41转化的细胞中，左末端区域至少存在50个拷贝，而在Ad40转化的细胞中存在2至3个拷贝。含有Ad41左末端区域的质粒在三种不同细胞系中的整合模式相似。