肠道腺病毒E1基因介导的细胞转化

Cellular transformation by E1 genes of enteric adenoviruses.

作者信息

Cousin C, Winter N, Gomes S A, D'Halluin J C

机构信息

Laboratoire de Virologie Moléculaire, INSERM U. 233, Lille, France.

出版信息

Virology. 1991 Mar;181(1):277-87. doi: 10.1016/0042-6822(91)90493-u.

DOI:10.1016/0042-6822(91)90493-u

PMID:1825253

Abstract

The ability of Ad40 and Ad41 E1A plus E1B genes to transform BRK cells was considerably lower than that of Ad5 and Ad12 corresponding genes. However, as for Ad5, the E1A genes of enteric adenoviruses could cooperate with an activated ras oncogene for full cell transformation and the Ad41 E1B could be complemented by E1A gene of Ad5 or Ad12 for cell transformation. Complementation studies suggested that the conserved region 1 of Ad41 E1A was responsible for this inefficient transformation. The Ad40- and Ad41-transformed cell lines exhibited a low level of major histocompatibility complex (MHC) class I antigens correlated to the low level of Ad12-transformed cells. Class I MHC antigen amounts expressed at the surface of the cells transformed by the weakly oncogenic Ad3 were between the high level of Ad5- and the low level of Ad12-transformed cells.

摘要

腺病毒40型（Ad40）和腺病毒41型（Ad41）的E1A加E1B基因转化BRK细胞的能力明显低于腺病毒5型（Ad5）和腺病毒12型（Ad12）的相应基因。然而，与Ad5一样，肠道腺病毒的E1A基因可与激活的ras癌基因协同作用以实现完全的细胞转化，并且Ad41的E1B可被Ad5或Ad12的E1A基因互补以实现细胞转化。互补研究表明，Ad41 E1A的保守区域1是这种低效转化的原因。Ad40和Ad41转化的细胞系表现出低水平的主要组织相容性复合体（MHC）I类抗原，这与Ad12转化细胞的低水平相关。由弱致癌性的Ad3转化的细胞表面表达的I类MHC抗原量介于Ad5转化细胞的高水平和Ad12转化细胞的低水平之间。