41型肠道腺病毒分离株：克隆、物理图谱及限制性酶切图谱的多样性
Enteric adenovirus type 41 isolates: cloning, physical maps and diversity in restriction enzyme cleavage pattern.
作者信息
Yamashita Y, Hotsubo T, Nakata S, Yamaguchi T, Sanekata T, Chiba S, Homma M, Fujinaga K
机构信息
Department of Molecular Biology, Cancer Research Institute, Hokkaido, Japan.
出版信息
Microbiol Immunol. 1992;36(12):1291-303. doi: 10.1111/j.1348-0421.1992.tb02131.x.
Adenovirus (Ad) type 40 and 41 DNAs were directly extracted from stool specimens of children with gastroenteritis. Two new strains of Ad41, Sanekata and Ehime strain, were cloned and their restriction maps were constructed. The left terminal end of the cloned Ad41 genome, EcoRI-E fragment of the Sanekata strain and EcoRI-F fragment of the Ehime strain, had transforming ability in rat 3Y1 cells. Only one of the 35 isolates of Ad40 tested showed a different restriction profile, while three different restriction profiles were found in DNAs from Ad41 isolates.
从患有肠胃炎的儿童粪便标本中直接提取40型和41型腺病毒（Ad）的DNA。克隆了两株新的Ad41毒株，即佐野型和爱媛型毒株，并构建了它们的限制性图谱。克隆的Ad41基因组的左末端，佐野型毒株的EcoRI-E片段和爱媛型毒株的EcoRI-F片段，在大鼠3Y1细胞中具有转化能力。在检测的35株Ad40分离株中，只有1株显示出不同的限制性图谱，而在Ad41分离株的DNA中发现了3种不同的限制性图谱。
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