Department of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram, 75000, Thailand.
Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Parasitol Res. 2024 Jul 23;123(7):283. doi: 10.1007/s00436-024-08295-x.
Mansonia uniformis (Diptera: Culicidae) is recognized as a vector of Brugia malayi and has been reported to transmit Wuchereria bancrofti, both causing lymphatic filariasis in humans. This study employed geometric morphometrics (GM) to investigate wing shape variation and analyzed genetic diversity through cytochrome c oxidase subunit 1 (COI) gene analyses in Ma. uniformis populations across Thailand. Wing GM analyses indicated significant differences in wing shape based on Mahalanobis distances among nearly all population pairs (p < 0.05), with no significant correlation between wing shape and geographic distance (r = 0.210, p > 0.05). Genetic analyses identified 63 haplotypes and 49 polymorphic sites, with the overall population exhibiting a nucleotide diversity of 0.006 (± 0.001) and a haplotype diversity of 0.912 (± 0.017). Deviations from neutrality, as indicated by Tajima's D and Fu's FS tests for the overall Ma. uniformis populations in Thailand, were statistically significant and negative, suggesting population expansion (both p < 0.05). Analysis of molecular variance revealed no significant genetic structure when all populations were categorized based on collection sites and geographic regions. However, significant differences in F values were observed between some populations. These findings enhance our understanding of the geographical and genetic factors influencing Ma. uniformis populations, which are crucial for developing effective control strategies in Thailand.
致倦库蚊（双翅目：蚊科）被认为是传播盘尾丝虫和班氏丝虫的媒介，这两种寄生虫都会导致人体淋巴丝虫病。本研究采用几何形态测量学（GM）分析泰国致倦库蚊种群的翅膀形状变化，并通过细胞色素 c 氧化酶亚基 1（COI）基因分析研究其遗传多样性。GM 分析表明，基于近所有种群之间的马氏距离，翅膀形状存在显著差异（p<0.05），而翅膀形状与地理距离之间无显著相关性（r=0.210，p>0.05）。遗传分析鉴定出 63 种单倍型和 49 个多态性位点，总体种群的核苷酸多样性为 0.006（±0.001），单倍型多样性为 0.912（±0.017）。泰国致倦库蚊总体种群的 Tajima's D 和 Fu's FS 检验均显示偏离中性，且呈显著负偏离，表明种群扩张（均 p<0.05）。当根据采集地点和地理区域对所有种群进行分类时，基于分子方差的分析未显示出显著的遗传结构。然而，一些种群之间的 F 值存在显著差异。这些发现增强了我们对影响致倦库蚊种群的地理和遗传因素的理解，这对于在泰国制定有效的控制策略至关重要。
