Polymers for Health and Biomaterials, IBMM, CNRS, ENSCM, University of Montpellier, 34090 Montpellier, France.
Department of Pharmacy, Nîmes University Hospital, 30900 Nimes, France.
Biomacromolecules. 2024 Aug 12;25(8):5098-5109. doi: 10.1021/acs.biomac.4c00495. Epub 2024 Jul 23.
The meniscus regeneration can present major challenges such as mimicking tissue microstructuration or triggering cell regeneration. In the case of lesions that require a personalized approach, photoprinting offers the possibility of designing resolutive biomaterial structures. The photo-cross-linkable ink composition determines the process ease and the final network properties. In this study, we designed a range of hybrid inks composed of gelatin(G) and 6-PLA arms(P) that were photo-cross-linked using tyramine groups. The photo-cross-linking efficiency, mechanical properties, degradation, and biological interactions of inks with different G/P mass ratios were studied. The G50P50 network properties were suitable for meniscus regeneration, with Young's modulus of 6.5 MPa, degradation in 2 months, and good cell proliferation. We then confirmed the potential of these inks to produce high-resolution microstructures by printing well-defined microstructures using two-photon polymerization. These hybrid inks offer new perspectives for biocompatible, degradable, and microstructured tissue engineering scaffold creation.
半月板再生可能会面临一些重大挑战，例如模拟组织微结构或触发细胞再生。对于需要个性化治疗的病变，光打印为设计有针对性的生物材料结构提供了可能。光交联墨水的组成决定了处理的难易程度和最终的网络性能。在这项研究中，我们设计了一系列由明胶(G)和 6-PLA 臂(P)组成的混合墨水，这些墨水使用酪氨酸基团进行光交联。研究了不同 G/P 质量比的墨水的光交联效率、机械性能、降解和生物相互作用。G50P50 网络性能适合半月板再生，杨氏模量为 6.5 MPa，两个月内降解，细胞增殖良好。然后，我们通过使用双光子聚合来打印定义良好的微结构，证实了这些墨水具有产生高分辨率微结构的潜力。这些混合墨水为创建具有生物相容性、可降解性和微结构的组织工程支架提供了新的前景。
