Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Korea.
Int J Mol Sci. 2020 May 11;21(9):3401. doi: 10.3390/ijms21093401.
In tissue engineering, biocompatible scaffolds are used as 3D cell niches to provide a similar environment to that of native tissue for seeded cells to regenerate the target tissue. When engineering bone tissue, high mechanical strength and calcium phosphate composition are essential factors to consider. In this study, we fabricated biocompatible composite scaffolds composed of synthetic polymers (polycaprolactone (PCL) and poly (vinyl alcohol) (PVA)), natural polymers (gelatin and collagen) and bioceramic (hydroxyapatite; HA) for bone tissue engineering. The synthetic polymers were used to enhance the mechanical properties of the composite scaffolds while the natural protein-based polymers were used to enhance various cellular activities, such as cell adhesion and proliferation. Meanwhile, the bioceramic was introduced to promote osteogenic differentiation. Composite scaffolds were evaluated for their physical characteristics, such as mechanical, swelling and protein absorbing properties as well as biological properties (cell proliferation, alkaline phosphatase (ALP) activities and calcium deposition) with human osteoblast-like cells (MG63). Consequently, incorporation of hydroxyapatite into the gelatin/PVA (C-GPH) scaffold showed 5-fold and 1.5-fold increase in calcium deposition and ALP activities, respectively compared to gelatin/PVA scaffold (C-GP). Moreover, compressive modulus also increased 1.8-fold. Integration of PCL core into gelatin/PVA/hydroxyapatite scaffold (C-PGPH) further amplified the compressive modulus 1.5-fold. In conclusion, the scaffold that is reinforced with HA particles and integrated with PCL core of the struts showed significant potential in field of bone tissue engineering.
在组织工程中，生物相容性支架被用作 3D 细胞小生境，为接种细胞提供类似于天然组织的环境，以再生目标组织。在工程骨组织时，高机械强度和钙磷组成是需要考虑的重要因素。在这项研究中，我们制备了由合成聚合物（聚己内酯 (PCL) 和聚乙烯醇 (PVA)）、天然聚合物（明胶和胶原蛋白）和生物陶瓷（羟基磷灰石；HA）组成的生物相容性复合支架用于骨组织工程。合成聚合物用于增强复合支架的机械性能，而天然蛋白基聚合物用于增强各种细胞活性，如细胞黏附和增殖。同时，引入生物陶瓷以促进成骨分化。通过人成骨样细胞 (MG63) 评估复合支架的物理特性，如机械性能、溶胀和蛋白质吸收特性以及生物学特性（细胞增殖、碱性磷酸酶 (ALP) 活性和钙沉积）。结果表明，与明胶/PVA 支架 (C-GP) 相比，羟基磷灰石掺入明胶/PVA（C-GPH）支架分别使钙沉积和 ALP 活性增加了 5 倍和 1.5 倍。此外，压缩模量也增加了 1.8 倍。将 PCL 芯整合到明胶/PVA/羟基磷灰石支架 (C-PGPH) 中进一步将压缩模量放大了 1.5 倍。总之，增强了 HA 颗粒并整合了支架支柱的 PCL 芯的支架在骨组织工程领域具有很大的潜力。
