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宫颈黏液电子显微镜检查方法的批判性评价

Critical evaluation of methods for electron microscopy of cervical mucus.

作者信息

Ulstein M, Myklebust R

出版信息

Acta Obstet Gynecol Scand. 1985;64(6):477-83. doi: 10.3109/00016348509156725.

DOI:10.3109/00016348509156725
PMID:3904320
Abstract

The ultrastructure of cervical mucus has been studied by scanning (SEM) and transmission (TEM) electron microscopy. The images differ according to the methods used for preparation; different patterns were found in the same sample when the freeze-drying and critical point-drying methods were used for SEM. When using TEM, the specimen appeared more homogeneous, with fine fibrils and granules. This accords with the theory that the cervical mucus consists of a randomly entangled fibrillar network. Electron microscopy of cervical mucus requires preparation procedures which involve considerable risk of creating artefacts. In our experience the TEM technique produces fewer artefacts and therefore gives pictures more true to the in vivo situation.

摘要

已通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)对宫颈黏液的超微结构进行了研究。根据制备方法的不同,图像也有所不同;在对同一样本进行扫描电镜观察时,采用冷冻干燥法和临界点干燥法会呈现出不同的模式。使用透射电镜时,标本看起来更均匀,有细小的纤维和颗粒。这与宫颈黏液由随机缠结的纤维网络组成的理论相符。宫颈黏液的电子显微镜检查需要一些制备程序,而这些程序存在产生伪像的较大风险。根据我们的经验,透射电镜技术产生的伪像较少,因此能提供更接近体内真实情况的图像。

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