Graduate Program in Neuropsychiatry and Behavioral Sciences, Federal University of Pernambuco, Recife, PE, Brazil.
Researcher in Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, Brazil.
Sci Rep. 2024 Jul 23;14(1):16883. doi: 10.1038/s41598-024-67887-w.
The state of Maternal Protein Malnutrition (MPM) is associated with several deleterious effects, including inflammatory processes and dysregulation in oxidative balance, which can promote neurodegeneration. On the other hand, it is known that aerobic exercise can promote systemic health benefits, combating numerous chronic diseases. Therefore, we evaluate the effect of aerobic exercise training (AET) on indicators of mitochondrial bioenergetics, oxidative balance, endoplasmic reticulum stress, and neurotrophic factor in the prefrontal cortex of malnourished juvenile Wistar rats. Pregnant Wistar rats were fed with a diet containing 17% or 8% casein during pregnancy and lactation. At 30 days of life, male offspring were divided into 4 groups: Low-Protein Control (LS), Low-Protein Trained (LT), Normoprotein Control (NS), and Normoprotein Trained (NT). The trained groups performed an AET for 4 weeks, 5 days a week, 1 h a day per session. At 60 days of life, the animals were sacrificed and the skeletal muscle, and prefrontal cortex (PFC) were removed to evaluate the oxidative metabolism markers and gene expression of ATF-6, GRP78, PERK and BDNF. Our results showed that MPM impairs oxidative metabolism associated with higher oxidative and reticulum stress. However, AET restored the levels of indicators of mitochondrial bioenergetics, in addition to promoting resilience to cellular stress. AET at moderate intensity for 4 weeks in young Wistar rats can act as a non-pharmacological intervention in fighting against the deleterious effects of a protein-restricted maternal diet.
母体蛋白质营养不良（MPM）状态与几种有害影响有关，包括炎症过程和氧化平衡失调，这可能促进神经退行性变。另一方面，已知有氧运动可以促进全身健康，对抗许多慢性疾病。因此，我们评估了有氧运动训练（AET）对营养不良幼年 Wistar 大鼠前额叶中线粒体生物能学、氧化平衡、内质网应激和神经营养因子指标的影响。怀孕的 Wistar 大鼠在怀孕期间和哺乳期喂食含有 17%或 8%酪蛋白的饮食。在 30 天大时，雄性后代分为 4 组：低蛋白对照组（LS）、低蛋白训练组（LT）、正常蛋白对照组（NS）和正常蛋白训练组（NT）。训练组进行了 4 周的 AET，每周 5 天，每天 1 小时。在 60 天大时，处死动物并取出骨骼肌和前额叶皮层（PFC），以评估氧化代谢标志物和 ATF-6、GRP78、PERK 和 BDNF 的基因表达。我们的结果表明，MPM 损害与更高的氧化和内质网应激相关的氧化代谢。然而，AET 恢复了线粒体生物能学指标的水平，除了促进细胞应激的弹性。在年轻的 Wistar 大鼠中进行 4 周中等强度的 AET 可以作为一种非药物干预措施，对抗蛋白质限制的母体饮食的有害影响。
