Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Department of General Practice, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Hypertens Res. 2024 Oct;47(10):2760-2772. doi: 10.1038/s41440-024-01791-8. Epub 2024 Jul 23.
Vascular remodeling is the main pathological process that causes the damage of the target organ of hypertension. Perivascular adipose tissue (PVAT) surrounds blood vessels and plays a key role in the pathogenesis of various cardiovascular diseases. This study aimed to investigate the effects of renal denervation (RDN) on hypertensive vascular remodeling and to elucidate the role of PVAT in this process. Male spontaneously hypertensive rat (SHR) and Wistar-Kyoto (WKY) rat were selected. Aortic vascular remodeling was evaluated using hematoxylin and eosin (H&E) staining and Masson's trichrome staining. Morphological changes in the PVAT were observed through H&E and Oil Red O staining. Dihydroethidium was used to measure oxidative stress levels in PVAT, while western blot analysis was used to determine the expression levels of proteins associated with vascular remodeling. The results showed that the aortic medial thickness, media thickness/lumen diameter, collagen volume fraction, and reactive oxygen species (ROS) level in PVAT were significantly higher in the SHR group than in the WKY group. The indexes mentioned above were lower in the SHR-RDN group than in the SHR group. H&E staining revealed that fat droplets in PVAT in the SHR-RDN group became smaller and browning occurred. Moreover, the protein expression of uncoupling protein-1 (UCP-1) and neuregulin 4 (Nrg4) was significantly increased in the SHR-RDN group. In addition, the expression of adiponectin increased and the expression of leptin decreased in the SHR-RDN group compared to the SHR group. In conclusion, RDN can relieve hypertensive vascular remodeling, which may be associated with PVAT.
血管重构是导致高血压靶器官损伤的主要病理过程。血管周围脂肪组织（PVAT）环绕血管，在各种心血管疾病的发病机制中发挥着关键作用。本研究旨在探讨肾脏去神经支配（RDN）对高血压血管重构的影响，并阐明 PVAT 在这一过程中的作用。选择雄性自发性高血压大鼠（SHR）和 Wistar-Kyoto（WKY）大鼠。采用苏木精和伊红（H&E）染色和 Masson 三色染色评估主动脉血管重构。通过 H&E 和油红 O 染色观察 PVAT 的形态变化。使用二氢乙锭测量 PVAT 中的氧化应激水平，并用 Western blot 分析测定与血管重构相关的蛋白表达水平。结果表明，与 WKY 组相比，SHR 组的主动脉中膜厚度、中膜厚度/腔直径、胶原体积分数和血管周围脂肪组织中的活性氧（ROS）水平显著升高。SHR-RDN 组的上述指标均低于 SHR 组。H&E 染色显示，SHR-RDN 组的 PVAT 中的脂肪滴变小，出现棕褐色。此外，SHR-RDN 组的解偶联蛋白 1（UCP-1）和神经调节蛋白 4（Nrg4）的蛋白表达显著增加。与 SHR 组相比，SHR-RDN 组的脂联素表达增加，瘦素表达减少。综上所述，RDN 可缓解高血压血管重构，这可能与 PVAT 有关。
