Wuhan University, Wuhan, China.
National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2024;2837:1-9. doi: 10.1007/978-1-0716-4027-2_1.
Hepatitis B, the leading cause of liver diseases worldwide, is a result of infection with hepatitis B virus (HBV). Due to its obligate intracellular life cycle, culture systems for efficient HBV replication are vital. Although basic and translational research on HBV has been performed for many years, conventional hepatocellular culture systems are not optimal. These studies have greatly benefited from recent improvements in cell culture models based on stem cell technology for HBV replication and infection studies. Here we describe a protocol for the differentiation of human stem cell-derived hepatocyte-like cells (HLCs) and subsequent HBV infection. HLCs are capable of expressing hepatocyte markers and host factors important for hepatic function maintenance. These cells fully support HBV infection and virus-host interactions. Stem cell-derived HLCs provide a new tool for antiviral drug screening and development.
乙型肝炎是全球肝脏疾病的主要原因，是由乙型肝炎病毒（HBV）感染引起的。由于其严格的细胞内生命周期，因此需要高效的乙型肝炎病毒复制的培养系统。尽管多年来一直在进行乙型肝炎的基础和转化研究，但常规的肝细胞培养系统并不理想。这些研究极大地受益于基于干细胞技术的乙型肝炎病毒复制和感染研究的细胞培养模型的最新改进。在这里，我们描述了一种人干细胞衍生的肝细胞样细胞（HLC）的分化方法，以及随后的乙型肝炎病毒感染方法。HLC 能够表达肝细胞标志物和宿主因子，这些对维持肝脏功能很重要。这些细胞完全支持乙型肝炎病毒的感染和病毒-宿主相互作用。干细胞衍生的 HLC 为抗病毒药物的筛选和开发提供了新工具。
