[DNA修复酶OGG1和MTH1抑制剂对非洲猪瘟病毒复制的影响]
[Effects of inhibitors of DNA repair enzymes OGG1 and MTH1 on the replication of African swine fever virus].
作者信息
Zhang Xiaoqiang, Fan Jie, Yang Jifei, Fu Jingsheng, Wu Mengli, Shao Chengxiang, Guan Guiquan, Yin Hong, Liu Zhijie, Niu Qingli
机构信息
African Swine Fever Regional Laboratory, China (Lanzhou), State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine and Biosafety, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China.
College of Medicine, Northwest Minzu University, Lanzhou 730030, Gansu, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2024 Jul 25;40(7):2136-2149. doi: 10.13345/j.cjb.230848.
African swine fever virus (ASFV), as a contagious viral pathogen, is responsible for the occurrence of African swine fever (ASF), a rapidly spreading and highly lethal disease. Since ASFV was introduced into China in 2018, it has been quickly spread to many provinces, which brought great challenges to the pig industry in China. Due to the limited knowledge about the pathogenesis of ASFV, neither vaccines nor antiviral drugs are available. We have found that ASFV infection can induce oxidative stress responses in cells, and DNA repair enzymes play a key role in this process. This study employed RNA interference, RT-qPCR, Western blotting, Hemadsorption (HAD), and flow cytometry to investigate the effects of the inhibitors of DNA repair enzymes OGG1 and MTH1 on ASFV replication and evaluated the anti-ASFV effects of the inhibitors. This study provides reference for the development of anti-viral drugs.
非洲猪瘟病毒（ASFV）作为一种传染性病毒病原体，是导致非洲猪瘟（ASF）发生的原因，非洲猪瘟是一种迅速传播且致死率很高的疾病。自2018年ASFV传入中国以来，它迅速传播至多个省份，给中国的养猪业带来了巨大挑战。由于对ASFV发病机制的了解有限，目前既没有疫苗也没有抗病毒药物。我们发现ASFV感染可诱导细胞中的氧化应激反应，并且DNA修复酶在此过程中起关键作用。本研究采用RNA干扰、RT-qPCR、蛋白质免疫印迹法、血细胞吸附（HAD）和流式细胞术来研究DNA修复酶OGG1和MTH1的抑制剂对ASFV复制的影响，并评估这些抑制剂的抗ASFV效果。本研究为抗病毒药物的开发提供了参考。
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