Wang Kang, Yu Pu, Chen Kong, Chen Min, Cai Yonghui, Wang Bingqing
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Jul 25;40(7):2346-2356. doi: 10.13345/j.cjb.230845.
To achieve rapid detection of enramycin in feed, we employed the competitive inhibition method to develop a colloidal gold immunochromatographic test strip based on the anti-enramycin A monoclonal antibody (anti-Er.A-mAb). Colloidal gold probes were prepared with a laboratory-prepared high-purity anti-Er.A-mAb. The effects of pH, antibody titer, and antigen concentration (test line) on the test strip performance were investigated. The colloidal gold test strip prepared with 8 μL potassium carbonate addition, 4 µg/mL antibody, 1.0 mg/mL antigen (test line), and 3 μL gold-labeled antibody showed acceptable specificity and a low limit of detection. The test strip showed the detection limit of 25 ng/mL for enramycin A, with a linear range of 25-300 ng/mL. The experiments on the feed with positive sample addition proved that the test strip had good repeatability and was more sensitive than high-performance liquid chromatography, being applicable for the rapid detection of enramycin in large batches of feed samples.
为实现对饲料中恩拉霉素的快速检测，我们采用竞争抑制法，基于抗恩拉霉素A单克隆抗体（抗Er.A-mAb）研制了一种胶体金免疫层析试纸条。用实验室制备的高纯度抗Er.A-mAb制备胶体金探针。研究了pH值、抗体效价和抗原浓度（检测线）对试纸条性能的影响。添加8 μL碳酸钾、4 µg/mL抗体、1.0 mg/mL抗原（检测线）和3 μL金标抗体制备的胶体金试纸条具有可接受的特异性和低检测限。该试纸条对恩拉霉素A的检测限为25 ng/mL，线性范围为25 - 300 ng/mL。添加阳性样品的饲料实验证明，该试纸条具有良好的重复性，且比高效液相色谱法更灵敏，适用于大批饲料样品中恩拉霉素的快速检测。
