Schully Kevin L, Voegtly Logan J, Rice Gregory K, Drumm Hannah, Fitzpatrick Maren C, Malagon Francisco, Shea April, Dong Ming, Oduro George, Robberts F J Lourens, Dartey Paul K A, Owusu-Ofori Alex, Clark Danielle V, Cer Regina Z, Bishop-Lilly Kimberly A
Austere Environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Command-Frederick, Ft. Detrick, MD, United States.
Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Command-Frederick, Ft. Detrick, MD, United States.
Front Microbiol. 2024 Jul 9;15:1401259. doi: 10.3389/fmicb.2024.1401259. eCollection 2024.
Melioidosis is a potentially severe disease caused by the gram-negative soil-dwelling bacterium called . The true breadth of the distribution of this tropical pathogen is starting to emerge with environmental and clinical isolates frequently characterized in new countries and regions. Even so, isolates, clinical cases, and genetic data from the continent of Africa remain scant. We previously confirmed the presence of in the environment of Ghana, unmasking a new area of endemicity for this pathogen. Here, we describe the genetic characteristics of isolates obtained from that environmental survey. Twenty-one isolates were subjected to whole genome sequencing and found to represent three discrete sequence types (ST), one of which was novel, and designated ST2058. Phylogenetic analysis places this novel isolate within a clade that includes genomes derived from the Americas, although it is closely related to a sub-clade that includes isolates from Africa. Importantly, phenotypic characterization demonstrates common features including API 20NE profiles and CPS to support existing diagnostics, and susceptibility to standard of care antibiotics often used in the clinical management of melioidosis. These findings add to our knowledge about the presence and distribution of in Africa and represent the first published genomes out of Ghana.
类鼻疽是一种由革兰氏阴性土壤细菌引起的潜在严重疾病。随着环境和临床分离株在新的国家和地区频繁被鉴定，这种热带病原体的真实分布范围开始显现。即便如此，来自非洲大陆的分离株、临床病例和基因数据仍然匮乏。我们之前证实了在加纳环境中存在该病原体，揭示了该病原体的一个新的地方性流行区域。在此，我们描述从该环境调查中获得的分离株的基因特征。对21株分离株进行全基因组测序，发现它们代表三种不同的序列类型（ST），其中一种是新的，被命名为ST2058。系统发育分析将这种新分离株置于一个包含来自美洲基因组的进化枝内，尽管它与一个包含来自非洲分离株的亚进化枝密切相关。重要的是，表型特征显示出共同特征，包括API 20NE图谱和荚膜多糖，以支持现有的诊断方法，以及对类鼻疽临床管理中常用的标准护理抗生素敏感。这些发现增加了我们对该病原体在非洲的存在和分布的了解，并代表了来自加纳的首批公开的基因组。
