Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil.
Laboratório de Imunobiologia das Doenças Infecciosas e Granulomatosas, Instituto de Biologia, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil.
Rev Bras Parasitol Vet. 2024 Jul 22;33(3):e002624. doi: 10.1590/S1984-29612024039. eCollection 2024.
The aim of this study was to determine the allergenic activity of components present in crude extracts of Pterobothrium crassicolle plerocerci (CPE) and blastocysts (CBE) obtained from Micropogonias furnieri in a murine model. Two groups of seven animals each received 50 µg of CPE or CBE on days 1, 35 and 120. Serum samples were tested by ELISA and Immunoblotting. Specific IgG and IgE levels were detected by ELISA, showing specific humoral responses for the primary immunization for both immunoglobulins and continuously growing titers for IgE. Positive Passive Cutaneous Anaphylaxis tests in rats sensitized with anti-CBE sera and tested by CBE, showed biologically, the allergenic activity of the extracts. The CPE and CBE showed some different recognition regions but both experimental groups recognized all regions of the extracts when tested for cross reactions, showing that CPE and CBE could share antigenic recognition sites.
本研究旨在确定从 Micropogonias furnieri 获得的 Pterobothrium crassicolle 中绦期幼虫（CPE）和囊尾蚴（CBE）粗提物中存在的成分在小鼠模型中的变应原活性。两组各有 7 只动物，分别在第 1 天、第 35 天和第 120 天接受 50 µg 的 CPE 或 CBE。通过 ELISA 和免疫印迹法检测血清样本。通过 ELISA 检测特异性 IgG 和 IgE 水平，显示两种免疫球蛋白的初次免疫均产生了特异性体液反应，并且 IgE 的滴度持续增加。用抗 CBE 血清致敏的大鼠进行的阳性被动皮肤过敏反应试验，用 CBE 进行测试，表明提取物具有生物学变应原活性。CPE 和 CBE 显示出一些不同的识别区域，但在进行交叉反应测试时，两个实验组均识别提取物的所有区域，表明 CPE 和 CBE 可能具有共同的抗原识别位点。
