Kutsenko N N, Kuzina Z A, Alekseeva L E, Iudelevich V I, Belousova N I
Antibiot Med Biotekhnol. 1985 Aug;30(8):592-5.
Products of kanamycin inactivation were identified chromatographically and radiometrically in E. coli 154, K. aerogenes 600 and P. vulgaris 7470. It was shown that the efficiency of kanamycin inactivation in the membrane fraction was 2 times higher than that in cytosol. A decrease in the culture resistance to kanamycin was observed, when the antibiotic was used in combination with the main proteins or phosphonites: a 16-64-fold decrease in the kanamycin MIC. Comparison of the data on the efficiency of the inactivation inhibition by intact cells and in acellular extracts suggests that the effect of protamine on this process is mediated by the cell membrane, whereas phosphonites can also directly interact with the enzymes inactivating the antibiotic.
在大肠杆菌154、产气克雷伯菌600和普通变形杆菌7470中，通过色谱法和放射性测量法鉴定了卡那霉素失活产物。结果表明，膜组分中卡那霉素失活效率比胞质溶胶中的高2倍。当抗生素与主要蛋白质或亚膦酸盐联合使用时，观察到培养物对卡那霉素的抗性降低：卡那霉素最低抑菌浓度降低了16至64倍。完整细胞和无细胞提取物对失活抑制效率的数据比较表明，鱼精蛋白对该过程的作用是由细胞膜介导的，而亚膦酸盐也可直接与使抗生素失活的酶相互作用。
