Klimniuk S I
Antibiotiki. 1984 Mar;29(3):182-4.
The effect of bile acids on antibiotic resistant strains of Staphylococcus, Proteus, E. coli and Ps. aeruginosa isolated from patients with different purulent inflammatory processes was studied. It was shown in vitro that glycocholic and deoxycholic acids lowered the resistance of the above microorganisms to some antibiotics. The exposure of the staphylococcal strains to deoxycholic or glycocholic acid lowered their resistance to streptomycin, neomycin and kanamycin on the average by 75-150, 125-500 and 55-60 times, respectively. The same tendency was observed in the penicillin and erythromycin resistant strains. A 2-hour incubation of E. coli strains in the presence of glycocholic or deoxycholic acid resulted in a decrease in the MIC of polymyxin B from 312.5 to 1.6 or 3.2 micrograms/ml, respectively. These acids increased bacterial sensitivity to streptomycin, chloramphenicol and neomycin by 8-16, 10-20 and 40-80 times, respectively. The effect of the bile acids on the resistant strains of E. coli and Proteus was less pronounced. It was shown that glycocholic and deoxycholic acids were able to inhibit the activity of staphylococcal beta-lactamase.
研究了胆汁酸对从患有不同化脓性炎症过程的患者中分离出的葡萄球菌、变形杆菌、大肠杆菌和铜绿假单胞菌的抗生素耐药菌株的影响。体外实验表明，甘胆酸和脱氧胆酸降低了上述微生物对某些抗生素的耐药性。葡萄球菌菌株暴露于脱氧胆酸或甘胆酸后，其对链霉素、新霉素和卡那霉素的耐药性平均分别降低了75 - 150倍、125 - 500倍和55 - 60倍。在耐青霉素和耐红霉素的菌株中也观察到了相同的趋势。大肠杆菌菌株在甘胆酸或脱氧胆酸存在下孵育2小时后，多粘菌素B的最低抑菌浓度分别从312.5微克/毫升降至1.6微克/毫升或3.2微克/毫升。这些酸分别使细菌对链霉素、氯霉素和新霉素的敏感性提高了8 - 16倍、10 - 20倍和40 - 80倍。胆汁酸对大肠杆菌和变形杆菌耐药菌株的作用不太明显。结果表明，甘胆酸和脱氧胆酸能够抑制葡萄球菌β-内酰胺酶的活性。
