Loewen P C, Switala J, Triggs-Raine B L
Arch Biochem Biophys. 1985 Nov 15;243(1):144-9. doi: 10.1016/0003-9861(85)90782-9.
Three strains of Escherichia coli differing only in the catalase locus mutated by transposon Tn10 were constructed. These strains produced only catalase HPI (katE::Tn10 and katF::Tn10 strains) or catalase HPII (katG::Tn10). HPI levels increased gradually about twofold during logarithmic growth but did not increase during growth into stationary phase in rich medium. HPII levels, which were initially threefold lower than HPI levels, did not change during logarithmic growth but did increase tenfold during growth into stationary phase. HPI levels increased in response to ascorbate or H2O2 being added to the medium but HPII levels did not. In minimal medium, any carbon source derived from the tricarboxylic acid cycle caused five- to tenfold higher HPII levels during logarithmic growth but had very little effect on HPI levels. Active electron transport did not affect either HPI or HPII levels.
构建了仅在被转座子Tn10突变的过氧化氢酶基因座上存在差异的三株大肠杆菌。这些菌株仅产生过氧化氢酶HPI（katE::Tn10和katF::Tn10菌株）或过氧化氢酶HPII（katG::Tn10）。在对数生长期，HPI水平逐渐增加约两倍，但在丰富培养基中生长进入稳定期时不增加。HPII水平最初比HPI水平低三倍，在对数生长期没有变化，但在生长进入稳定期时增加了十倍。向培养基中添加抗坏血酸或H2O2会使HPI水平升高，但HPII水平不会。在基本培养基中，任何源自三羧酸循环的碳源在对数生长期会使HPII水平提高五到十倍，但对HPI水平影响很小。活跃的电子传递对HPI或HPII水平均无影响。
