National Microbiology Laboratory, Winnipeg, MB, R3E 3R2, Canada.
Laboratoire de santé publique du Québec, Ste-Anne-de-Bellevue, Québec, Canada.
Eur J Clin Microbiol Infect Dis. 2024 Oct;43(10):2047-2051. doi: 10.1007/s10096-024-04907-7. Epub 2024 Jul 24.
Carbapenem-resistance in Enterobacter spp due to acquisition of mobile carbapenemases is of concern. An Enterobacter spp grew on ChromID CARBA medium and was positive for the mCIM carbapenemase detection assay. Susceptibility testing showed resistance to aztreonam and reduced susceptibility to imipenem. Conventional PCR using FRI primers detected a bla gene. Whole genome sequencing reveled a new variant; bla was closest in sequence to bla differing by 13 amino acids and was found on a unique 110Kb IncR plasmid. Given the intrinsic nature of Enterobacter spp. to be carbapenem non-susceptible, blaFRI-types may be under reported globally.
肠杆菌属由于获得移动碳青霉烯酶而导致的碳青霉烯耐药性令人担忧。一株肠杆菌属在 ChromID CARBA 培养基上生长，mCIM 碳青霉烯酶检测呈阳性。药敏试验显示对氨曲南耐药，对亚胺培南的敏感性降低。使用 FRI 引物的常规 PCR 检测到 bla 基因。全基因组测序揭示了一种新的变体；bla 与 bla 序列最接近，仅相差 13 个氨基酸，位于一个独特的 110Kb IncR 质粒上。鉴于肠杆菌属的固有性质，碳青霉烯类药物的不敏感性，blaFRI 型可能在全球范围内报告不足。
