National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Laboratoire de santé Publique du Québec, Ste-Anne-de-Bellevue, Canada.
Microb Drug Resist. 2023 Feb;29(2):47-50. doi: 10.1089/mdr.2022.0123.
This study investigated the mechanism of carbapenem resistance in an complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of (ST1639) and the presence of located on a 148kb IncFII(Yp) plasmid. This is the first occurrence of a clinical isolate harboring the FRI-8 carbapenemase and the second occurrence of FRI in Canada. This study highlights the need to use both WGS and phenotypic screening methods for detection of carbapenemase-producing strains if we consider the growing diversity of carbapenemases.
本研究通过改良碳青霉烯灭活法（mCIM）检测到一株复杂阳性但罗氏 neo-rapid Carb 试剂盒、β CARBA 和常见碳青霉烯酶基因（KPC、NDM、OXA-48、IMP、VIM、GES 和 IMI/NMC）常规 PCR 检测为阴性的产碳青霉烯酶菌株的耐药机制。使用全基因组测序（WGS）数据，我们确认了 ST1639 的鉴定和位于 148kb IncFII(Yp) 质粒上的 FRI-8 碳青霉烯酶的存在。这是加拿大首次出现携带 FRI-8 碳青霉烯酶的临床分离株，也是加拿大第二次出现 FRI。本研究强调，如果我们考虑到碳青霉烯酶的多样性不断增加，那么在检测产碳青霉烯酶菌株时，需要同时使用 WGS 和表型筛选方法。
